Here, we propose a novel imaging method, which is called image scanning difference microscopy (ISDM), for superresolution imaging. In ISDM, we implement a detector array composed of 19 avalanche photodiodes (APD) rather than single‐point detector in standard confocal microscopy for reconstructing superresolved images with higher signal‐to‐noise ratio (SNR). Combining with our former proposed fluorescence emission difference (FED) method, we have achieved a lateral resolution of 111 nm (∼λ/6) without the damage of image quality, the highest FED resolution to the best of our knowledge. With its simple setup and remarkable performance, we believe that ISDM can become a versatile observation tool in biology and other fundamental studies.

中文翻译：

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图像扫描差分显微镜

在这里，我们提出了一种新的成像方法，称为图像扫描差分显微镜 (ISDM)，用于超分辨率成像。在 ISDM 中，我们实现了由 19 个雪崩光电二极管 (APD) 组成的探测器阵列，而不是标准共聚焦显微镜中的单点探测器，用于重建具有更高信噪比 (SNR) 的超分辨图像。结合我们之前提出的荧光发射差异 (FED) 方法，我们在不损害图像质量的情况下实现了 111 nm (∼λ/6) 的横向分辨率，这是我们所知的最高 FED 分辨率。凭借其简单的设置和卓越的性能，我们相信 ISDM 可以成为生物学和其他基础研究中的多功能观察工具。