Environmental quality guideline values and remediation targets, specific to Antarctic ecosystems, are required for the risk assessment and remediation of contaminated sites in Antarctica. Ecotoxicological testing with Antarctic soil organisms is fundamental in determining reliable contaminant effect threshold concentrations. The present study describes the development of optimal culturing techniques and aqueous toxicity test procedures for an endemic Antarctic soil nematode, Plectus murrayi, which lives within interstitial waters between soil particles. Toxicity tests were of extended duration to account for the species' physiology and life-history characteristics. Plectus murrayi was sensitive to aqueous copper with a 50% effective concentration for egg-hatching success of 139 µg/L. Hatched juveniles that were first exposed to copper as eggs appeared to be less sensitive than those first exposed at the hatched J2 stage, indicating a potential protective effect of the egg. Sensitivity of juveniles to copper increased with exposure duration, with 50% lethal concentrations of 478 and 117 µg/L at 21 and 28 d, respectively. The present study describes new methods for the application of an environmentally relevant test species to the risk assessment of contaminants in Antarctic soil and provides the first estimates of sensitivity to a toxicant for an Antarctic terrestrial microinvertebrate. Environ Toxicol Chem 2020;39:482-491. © 2019 SETAC.

中文翻译：

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对铜的敏感性以及南极陆生线虫Plectus murrayi的培养和毒性测试程序的发展。

南极生态系统特有的环境质量准则值和补救目标是对南极洲受污染场地进行风险评估和补救所必需的。用南极土壤生物进行生态毒理学测试是确定可靠的污染物影响阈值浓度的基础。本研究描述了一种生活在土壤颗粒之间的间质水中的地方性南极土壤线虫Plectus murrayi的最佳培养技术和水毒性测试程序的发展。毒性测试的持续时间较长，以说明该物种的生理和生活史特征。Plectus murrayi对铜水溶液敏感，有效浓度为50％的卵孵化成功率为139 µg / L。刚孵化为卵的刚孵化的幼虫似乎不如刚孵化的J2阶段刚孵出的幼虫敏感，表明该卵具有潜在的保护作用。幼虫对铜的敏感性随接触时间的延长而增加，在21和28 d时，致死浓度的50％分别为478和117 µg / L。本研究描述了一种新的方法，用于将与环境有关的测试物种应用于南极土壤中污染物的风险评估，并提供了对南极陆生微脊椎动物无毒物质敏感性的第一个估计值。Environ Toxicol Chem 2020； 39：482-491。©2019 SETAC。幼虫对铜的敏感性随接触时间的延长而增加，在21和28 d时，致死浓度的50％分别为478和117 µg / L。本研究描述了一种新的方法，用于将与环境有关的测试物种应用于南极土壤中污染物的风险评估，并提供了对南极陆地无脊椎动物无毒物敏感性的第一个估计值。Environ Toxicol Chem 2020； 39：482-491。©2019 SETAC。幼虫对铜的敏感性随接触时间的延长而增加，在21和28 d时，致死浓度的50％分别为478和117 µg / L。本研究描述了一种新的方法，用于将与环境有关的测试物种应用于南极土壤中污染物的风险评估，并提供了对南极陆生微脊椎动物无毒物质敏感性的第一个估计值。Environ Toxicol Chem 2020； 39：482-491。©2019 SETAC。39：482-491。©2019 SETAC。39：482-491。©2019 SETAC。