Steroid hormones play a critical role in maintaining the homeostasis of human metabolism. Urine as a noninvasive sample has been extensively used in clinical diagnosis for hormones homeostasis. In this study, the simultaneous characterization of fourteen hormones in urine was performed based on ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPHLC/ESI(+)-MS/MS) with multiple reaction monitoring in the positive ionization mode. The target hormones were cortisone, cortisol, 11-deoxycortisol, aldosterone, corticosterone, 11-deoxycorticosterone, progesterone, 17-OH-progesterone, pregnenolone, estrone, estradiol, estriol, testosterone and dehydreopiandrosterone. β-Glucuronidase/sulfatase deconjugation and liquid-liquid extraction (LLE) were conducted for the determination of urinary hormones (free + conjugated forms). The limits of detection (LODs) ranged from 0.2 ng/mL (11-deoxycortisol and testosterone) to 1 ng/mL (cortisone). The extraction recovery of the targeted compounds ranged from 87% to 127%, indicating sufficient extraction efficiency for the LLE process. Intraday precision was below 10% and the accuracy ranged from 84% to 122%. The profiling analysis of hormones in urine samples helps to understand the metabolic state of biological systems and can be employed as a diagnostic tool in diseases developed by endocrine-disrupted systems.

中文翻译：

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超高效液相色谱/三重四极杆质谱法测定人尿中的激素。

类固醇激素在维持人体新陈代谢的稳态中起着关键作用。尿液作为一种非侵入性样品已广泛用于激素稳态的临床诊断。在这项研究中，基于超高效液相色谱/电喷雾串联质谱法（UPHLC / ESI（+）-MS / MS）进行尿液中14种激素的同时表征，并以正电离模式进行多反应监测。目标激素为可的松，皮质醇，11-脱氧皮质醇，醛固酮，皮质酮，11-脱氧皮质酮，孕酮，17-OH-孕酮，孕烯醇酮，雌酮，雌二醇，雌三醇，睾丸激素和脱水戊二酮。进行了β-葡萄糖醛酸苷酶/硫酸酯酶的解偶联和液-液萃取（LLE），以测定尿激素（游离+结合形式）。检出限（LOD）为0.2 ng / mL（11-脱氧皮质醇和睾丸激素）至1 ng / mL（可的松）。目标化合物的提取回收率在87％至127％之间，表明LLE工艺具有足够的提取效率。日内精确度低于10％，精确度介于84％至122％之间。尿液样本中的激素分布图分析有助于了解生物系统的代谢状态，并可作为由内分泌紊乱的系统发展而来的疾病的诊断工具。目标化合物的提取回收率在87％至127％之间，表明LLE工艺具有足够的提取效率。日内精确度低于10％，精确度介于84％至122％之间。尿液样本中的激素分布图分析有助于了解生物系统的代谢状态，并可作为由内分泌紊乱的系统发展而来的疾病的诊断工具。目标化合物的提取回收率在87％至127％之间，表明LLE工艺具有足够的提取效率。日内精确度低于10％，精确度介于84％至122％之间。尿液样本中的激素分布图分析有助于了解生物系统的代谢状态，并可作为由内分泌紊乱的系统发展而来的疾病的诊断工具。