Prepubertal Swiss albino mice of both sex were administered with first-line anti-tuberculosis drugs (ATDs) viz; rifampicin, isoniazid, pyrazinamide, streptomycin and ethambutol intraperitoneally, for 4 weeks. Two weeks after the completion of treatment, male mice were sacrificed to collect caudal spermatozoa and female mice were superovulated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) to collect metaphase II (MII) oocytes from oviduct. Administration of ATDs not only decreased the count, motility and, nuclear maturity and also, increased the head abnormalities, mitochondrial damage and DNA damage in epididymal spermatozoa. Reduction in number of ovulated oocytes, increased degeneration rate and altered distribution pattern of cytoplasmic organelles was observed in oocytes of female mice. Presence of ATDs in in vitro maturation (IVM) medium increased abnormalities in meiotic resulted in abnormal spindle organization (except ethambutol) without affecting nuclear maturation. In conclusion, the result of this study indicates that ATDs have considerable adverse effects on the functional competence of male and female gametes, however, with varied degree of toxicity.

对两性的青春期前瑞士白化病小鼠分别给予一线抗结核药物（ATD）治疗；利福平，异烟肼，吡嗪酰胺，链霉素和乙胺丁醇腹膜内给药，持续4周。治疗结束两周后，处死雄性小鼠以收集尾部精子，雌性小鼠用孕母马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）超排卵，以从输卵管中收集中期II（MII）卵母细胞。施用ATD不仅减少了附睾精子的数量，运动性和核成熟度，而且还增加了头部异常，线粒体损伤和DNA损伤。在雌性小鼠的卵母细胞中观察到排卵卵母细胞数量减少，变性速率增加和细胞质细胞器分布模式改变。ATD的存在体外成熟（IVM）培养基减数分裂异常增加，导致纺锤体组织异常（乙胺丁醇除外），而不会影响核成熟。总而言之，这项研究的结果表明，ATD对雄性和雌性配子的功能能力具有相当大的不利影响，但是具有不同程度的毒性。