Abstract In this study, the potential of Thraustochytrium sp. BM2 for cost-effective lipid production was investigated. Lipid content and productivity of BM2 reached 76% and 37 mg/L/h when corn steep liquor (12.5 g/L) and glycerol (10 g/L) was used as nitrogen and carbon source respectively. Supplementing an optimal amount of sea salt (2% w/v) further enhanced the lipid content to 79%. The inoculum size and age were also evaluated for their effect on lipid accumulation. A 48-h pre-culture at 0.15 g/L loading further improved the lipid productivity to 43.86 mg/L/h (or 1.052 g/L/d), which is a 340% increase when compared with the control test. Thus, Thraustochytrium sp. BM2 strain could serve as a low-cost and high-lipid-yield heterotrophic lipid producer using glycerol (a by-product of biodiesel manufacturing process) as carbon source and an inexpensive nitrogen source (i.e., corn steep liquor).

中文翻译：

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使用异养海洋微藻 Thraustochytrium sp. 增强微藻脂质的产生。BM2

摘要 在这项研究中，破囊壶菌属的潜力。研究了用于具有成本效益的脂质生产的 BM2。当玉米浆(12.5 g/L)和甘油(10 g/L)分别作为氮源和碳源时，BM2的脂质含量和生产率分别达到76%和37 mg/L/h。补充最佳量的海盐 (2% w/v) 进一步将脂质含量提高到 79%。还评估了接种物的大小和年龄对脂质积累的影响。以 0.15 g/L 负载量进行 48 小时预培养进一步将脂质生产率提高到 43.86 mg/L/h（或 1.052 g/L/d），与对照测试相比增加了 340%。因此，破囊壶菌属。