Abstract A matrix-free laser desorption/ionization mass spectrometry (LDI-MS) approach was developed using electrospun composite nanofibers with controllable size, morphology and composition. Composite polyvinylpyrrolidone (PVP) nanofibers with added nanoparticles to enhance the absorption of laser energy absorber were studied. Fractal dimensional analysis was conducted to evaluate the electrospinning process for the first time, which provided useful information on the repeatability of nanofiber production. The performance of the nanofiber-assisted laser desorption/ionization method was investigated through characterization of small drug molecules and synthetic oligomers. Homogeneous sample distribution was achieved by eliminating the “sweet spot”, resulting in good reproducibility. Mass spectra features clean background, that is especially beneficial for interpretation of small molecules. High sensitivity for small drug molecules and synthetic oligomers was obtained with limits of detection limits down to the low attomolar range. The combination of composite nanofibers with LDI-MS is a versatile and sensitive approach for detection and characterization of analytes with broad range of molecular weight.

中文翻译：

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聚乙烯吡咯烷酮复合纳米纤维作为表面辅助激光解吸/电离质谱的有效基材

摘要 使用尺寸、形态和成分可控的电纺复合纳米纤维开发了一种无基质激光解吸/电离质谱 (LDI-MS) 方法。研究了添加纳米粒子以增强激光能量吸收剂吸收的复合聚乙烯吡咯烷酮 (PVP) 纳米纤维。首次进行了分形维数分析以评估静电纺丝过程，这为纳米纤维生产的可重复性提供了有用的信息。通过对药物小分子和合成低聚物的表征，研究了纳米纤维辅助激光解吸/电离方法的性能。通过消除“最佳点”实现了均匀的样品分布，从而获得了良好的重现性。质谱具有干净的背景，这对小分子的解释尤其有益。对小药物分子和合成低聚物具有高灵敏度，检测限低至低阿托摩尔范围。复合纳米纤维与 LDI-MS 的结合是一种通用且灵敏的方法，可用于检测和表征具有广泛分子量范围的分析物。