In the present study, the biochemical responses and antioxidant enzymes activity of the Dunaliella salina, a green microalga, to the interaction of silver nanoparticles (AgNPs) and salicylic acid (SA) were investigated. Algal suspensions in the phase of logarithmic growth were subjected to the concentrations of 0, 5, 15, and 25 pM AgNPs with or without 1 mM SA. AgNPs level of 25 pM declined cell division but highly accumulated levels of chlorophyll, β-carotene, proteins, free amino acid, carbohydrates, and hydrogen peroxide, which was associated with enhanced the activity of proteolysis, lipid peroxidation, and antioxidant enzymes. SA-treated cells at 25 pM AgNPs improved cell growth but declined the activities of antioxidant enzymes and proteolytic along with a lower accumulation of metabolites except β-carotene relative to untreated controls. These results suggest that AgNPs treatment induce oxidative stress in D. salina cells, which tolerated by alga through the metabolic modifications and accumulating β-carotene, while SA induces AgNPs tolerance by the mechanisms that direct carbon flux to growth and β-carotene biosynthesis rather than the antioxidant enzymes or osmoprotectant metabolites.

中文翻译：

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在水杨酸处理下，绿色微藻杜氏盐藻对银纳米颗粒诱导的氧化应激的代谢反应。

在本研究中，研究了绿色微藻杜氏藻对银纳米颗粒（AgNPs）和水杨酸（SA）相互作用的生化响应和抗氧化酶活性。对数生长期的藻类悬浮液的浓度分别为有，无1 mM SA的0、5、15和25 pM AgNP。AgNPs的水平为25 pM，可降低细胞分裂，但叶绿素，β-胡萝卜素，蛋白质，游离氨基酸，碳水化合物和过氧化氢的积累水平较高，这与蛋白水解，脂质过氧化和抗氧化酶的活性增强有关。与未经处理的对照组相比，经SA处理的25 pM AgNPs的细胞改善了细胞生长，但降低了抗氧化酶和蛋白水解的活性，并降低了除β-胡萝卜素以外的代谢产物的积累。