Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is a devastating disease of rapeseed (Brassica napus L.). To date, the genetic mechanisms of rapeseed' interactions with S. sclerotiorum are not fully understood, and molecular-based breeding is still the most effective control strategy for this disease. Here, Arabidopsis thaliana GDSL1 was characterized as an extracellular GDSL lipase gene functioning in Sclerotinia resistance. Loss of AtGDSL1 function resulted in enhanced susceptibility to S. sclerotiorum. Conversely, overexpression of AtGDSL1 in B. napus enhanced resistance, which was associated with increased reactive oxygen species (ROS) and salicylic acid (SA) levels, and reduced jasmonic acid levels. In addition, AtGDSL1 can cause an increase in lipid precursor phosphatidic acid levels, which may lead to the activation of downstream ROS/SA defence-related pathways. However, the rapeseed BnGDSL1 with highest sequence similarity to AtGDSL1 had no effect on SSR resistance. A candidate gene association study revealed that only one AtGDSL1 homolog from rapeseed, BnaC07g35650D (BnGLIP1), significantly contributed to resistance traits in a natural B. napus population, and the resistance function was also confirmed by a transient expression assay in tobacco leaves. Moreover, genomic analyses revealed that BnGLIP1 locus was embedded in a selected region associated with SSR resistance during the breeding process, and its elite allele type belonged to a minor allele in the population. Thus, BnGLIP1 is the functional equivalent of AtGDSL1 and has a broad application in rapeseed S. sclerotiorum-resistance breeding.

中文翻译：

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拟南芥GDSL1的过表达增强了油菜菌核盘菌的抗药性，并增强了其在甘蓝型油菜中的同源性的功能鉴定。

核盘菌核盘菌引起的核盘菌茎腐病（SSR）是油菜（Brassica napus L.）的毁灭性疾病。迄今为止，尚未完全了解油菜与核盘菌的遗传机制，基于分子的育种仍然是该病最有效的控制策略。在这里，拟南芥GDSL1被表征为在菌核病抗性中发挥功能的细胞外GDSL脂肪酶基因。AtGDSL1功能的丧失导致对核盘菌的敏感性增加。相反，在甘蓝型油菜中AtGDSL1的过表达增强了抗性，这与活性氧（ROS）和水杨酸（SA）含量增加以及茉莉酸含量降低有关。此外，AtGDSL1会导致脂质前体磷脂酸水平升高，这可能会导致下游ROS / SA防御相关途径的激活。但是，与AtGDSL1具有最高序列相似性的油菜籽BnGDSL1对SSR抗性没有影响。一项候选基因关联研究表明，只有一个来自油菜籽的AtGDSL1同源物BnaC07g35650D（BnGLIP1）显着促进了自然双歧甘蓝种群的抗性特性，而且抗性功能也通过烟草叶中的瞬时表达分析得以证实。此外，基因组分析表明，BnGLIP1基因座在育种过程中嵌入了与SSR抗性相关的选定区域，其优良等位基因类型属于种群中的次要等位基因。因此，BnGLIP1是AtGDSL1的功能等同物，在油菜菌核盘菌抗性育种中具有广泛的应用。