Microglial expression of GAT-1 in the cerebral cortex.,Glia

当前位置： X-MOL 学术 › Glia › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Microglial expression of GAT-1 in the cerebral cortex.
Glia ( IF 5.4 ) Pub Date : 2019-11-06 , DOI: 10.1002/glia.23745
Giorgia Fattorini _{1,

2} , Myriam Catalano ₃ , Marcello Melone _{1,

2} , Carmela Serpe ₃ , Silvia Bassi ₁ , Cristina Limatola _{3,

4} , Fiorenzo Conti _{1,

2}

Affiliation

Microglial cells are the immune cells of the brain that, by sensing the microenvironment, permit a correct brain development and function. They communicate with other glial cells and with neurons, releasing and responding to a number of molecules that exert effects on surrounding cells. Among these, neurotransmitters and, in particular, gamma-aminobutyric acid (GABA) has recently gained interest in this context. We demonstrated the expression of GABA transporter 1 (GAT-1) in microglial cells both in soma and cell processes. We show that microglial cell treatment with 1,2,5,6-tetrahydro-1-[2-[[(diphenylmethylene)amino]oxy]ethyl]-3-pyridinecarboxylic acid hydrochloride (NNC-711), a potent and selective GAT-1 inhibitor, significantly reduced Na+ -dependent GABA uptake. On the other hand, GABA uptake was significantly increased by cell treatment with (S)-1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-3-piperidinecarboxylic acid (SNAP-5114), a GAT-2/3 inhibitor, and this effect was completely blocked by the botulinum toxin BoNT/C1, that specifically cleaves and inactives syntaxin 1A (STX1A). Overall, these findings show that microglial cells express GAT-1 and indicate that STX1A plays an important role in the regulation of GAT-1-dependent GABA uptake in microglia.

中文翻译：

GAT-1在大脑皮层的小胶质细胞表达。

小胶质细胞是大脑的免疫细胞，通过感知微环境，可以使大脑正确发育和发挥功能。它们与其他神经胶质细胞和神经元进行通讯，释放并响应对周围细胞产生影响的许多分子。其中，神经递质，尤其是γ-氨基丁酸（GABA）最近在这种情况下引起了人们的兴趣。我们证明了GABA转运蛋白1（GAT-1）在体细胞和细胞过程中在小胶质细胞中的表达。我们显示1,2,5,6-四氢-1- [2-[[[（[（二苯甲基）氨基]氧基]乙基] -3-吡啶羧酸盐酸盐（NNC-711），有效和选择性的GAT小胶质细胞治疗。 -1抑制剂可显着降低Na +依赖性GABA的吸收。另一方面，通过用GAT-2 / 3抑制剂（S）-1- [2- [三[4-（三甲氧基苯基）甲氧基]乙基] -3-哌啶羧酸（SNAP-5114）处理，GABA的摄取显着增加。这种作用完全被肉毒杆菌毒素BoNT / C1阻断，该酶特异性裂解和失活1A（STX1A）语法。总体而言，这些发现表明小胶质细胞表达GAT-1，并表明STX1A在小胶质细胞中依赖GAT-1的GABA吸收调控中起着重要作用。

更新日期：2019-11-06

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11