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Characterization of glycerophospholipid molecular species in muscles from three species of cephalopods by direct infusion-tandem mass spectrometry.
Chemistry and Physics of Lipids ( IF 3.4 ) Pub Date : 2019-11-06 , DOI: 10.1016/j.chemphyslip.2019.104848
Yan Shen 1 , Hong-Kai Xie 2 , Zhong-Yuan Liu 1 , Ting Lu 1 , Zhuo-Liang Yu 1 , Li-Hua Zhang 1 , Da-Yong Zhou 3 , Tong Wang 4
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More than 200 molecular species of glycerophospholipids (GP) including glycerophosphocholine (GPC), glycerophosphoethanolamine (GPE), glycerophosphoserine (GPS), lysoglycerophosphocholine (LGPC), lysoglycerophosphoethanolamine (LGPE) and lysoglycerophosphoserine (LGPS), as well as 18 kinds of sphingomyelin (SM) were characterized by using a direct infusion-tandem mass (MS/MS) spectrometry method for lipids from the muscles of cephalopods Sepiella maindroni, Octopus ocellatus and Loligo chinensis for the first time. The majority of the GP molecular species contained long-chain omega-3 polyunsaturated fatty acids (n-3 LC-PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Therefore, cephalopods can be a good possible source of dietary GP carrying n-3 LC-PUFA. The total lipids were composed of phospholipid (PL, 72.29-83.32 wt% of total lipids), cholesterol (12.70-23.60 wt% of total lipids), triacylglycerol (1.86-2.93 wt% of total lipids), diacylglycerol (0.15-1.09 wt% of total lipids), monoacylglycerol (0.06-0.18 wt% of total lipids) and free fatty acid (0.72-1.86 wt% of total lipids). For PL, phosphatidylcholine (44.47-62.30 mol%), phosphatidylethanolamine (22.57-39.08 mol%), phosphatidylserine (6.15-10.18 mol%), phosphatidylglycerol (0.68-3.11 mol%), phosphatidylinositol (2.41-7.15 mol%) and lysophosphatidylcholine (1.84-5.24 mol%) were detected. Furthermore, the total lipids from the muscles of cephalopods Sepiella maindroni, Octopus ocellatus and Loligo chinensis contained 41.80-50.02 mol% of saturated fatty acids, 11.53-21.54 mol% of monounsaturated fatty acids and 36.67-40.82 mol% of PUFA, whilst DHA (15.25-26.71 mol%) and EPA (6.29-16.57 mol%) were found to account for the majority of the PUFA. With these data presented, cephalopod muscle can be considered as a healthy food for humans.

中文翻译:

通过直接输注串联质谱法表征三种头足类动物肌肉中的甘油磷脂分子种类。

超过200个分子种类的甘油磷脂(GP),包括甘油磷酸胆碱(GPC),甘油磷酸乙醇胺(GPE),甘油磷酸丝氨酸(GPS),溶血甘油磷酸胆碱(LGPC),溶血甘油磷酸乙醇胺(LGPE)和溶血甘油磷酸丝氨酸(LGPS)(以及18种sphlyglycerophosphining(LGPS)) )首次使用直接输注串联质谱(MS / MS)方法对头足类Sepiella maindroni,Octopus ocellatus和Loligo chinensis的肌肉中的脂质进行了表征。GP分子中的大多数都包含长链omega-3多不饱和脂肪酸(n-3 LC-PUFA),尤其是二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)。因此,头足类可能是携带n-3 LC-PUFA的饮食GP的良好来源。总脂质由磷脂组成(PL,72.29-83。占总脂质的32 wt%),胆固醇(占总脂质的12.70-23.60 wt%),三酰甘油(占总脂质的1.86-2.93 wt%),二酰基甘油(占总脂质的0.15-1.09 wt%),单酰基甘油(0.06-0.18 wt。总脂质的%)和游离脂肪酸(总脂质的0.72-1.86wt%)。对于PL,磷脂酰胆碱(44.47-62.30 mol%),磷脂酰乙醇胺(22.57-39.08 mol%),磷脂酰丝氨酸(6.15-10.18 mol%),磷脂酰甘油(0.68-3.11 mol%),磷脂酰肌醇(2.41-7.15 mol%)和检测到1.84-5.24mol%。此外,头足类Sepiella maindroni,Octopus ocellatus和Loligo chinensis的肌肉中的总脂质包含41.80-50.02 mol%的饱和脂肪酸,11.53-21.54 mol%的单不饱和脂肪酸和36.67-40.82 mol%的PUFA,而DHA( 15.25-26.71 mol%)和EPA(6.29-16。发现57摩尔%占PUFA的大部分。根据这些数据,头足类肌肉可以被认为是对人类健康的食品。
更新日期:2019-11-06
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