当前位置：
X-MOL 学术
›
Plant Cell
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A Large Transposon Insertion in the stiff1 Promoter Increases Stalk Strength in Maize.
The Plant Cell ( IF 10.0 ) Pub Date : 2019-11-04 , DOI: 10.1105/tpc.19.00486 Zhihai Zhang 1 , Xuan Zhang 1 , Zhelong Lin 1 , Jian Wang 1 , Hangqin Liu 1 , Leina Zhou 1 , Shuyang Zhong 1 , Yan Li 1 , Can Zhu 1 , Jinsheng Lai 1 , Xianran Li 2 , Jianming Yu 2 , Zhongwei Lin 3
The Plant Cell ( IF 10.0 ) Pub Date : 2019-11-04 , DOI: 10.1105/tpc.19.00486 Zhihai Zhang 1 , Xuan Zhang 1 , Zhelong Lin 1 , Jian Wang 1 , Hangqin Liu 1 , Leina Zhou 1 , Shuyang Zhong 1 , Yan Li 1 , Can Zhu 1 , Jinsheng Lai 1 , Xianran Li 2 , Jianming Yu 2 , Zhongwei Lin 3
Affiliation
|
Stalk lodging, which is generally determined by stalk strength, results in considerable yield loss and has become a primary threat to maize (Zea mays) yield under high-density planting. However, the molecular genetic basis of maize stalk strength remains unclear, and improvement methods remain inefficient. Here, we combined map-based cloning and association mapping and identified the gene stiff1 underlying a major quantitative trait locus for stalk strength in maize. A 27.2-kb transposable element insertion was present in the promoter of the stiff1 gene, which encodes an F-box domain protein. This transposable element insertion repressed the transcription of stiff1, leading to the increased cellulose and lignin contents in the cell wall and consequently greater stalk strength. Furthermore, a precisely edited allele of stiff1 generated through the CRISPR/Cas9 system resulted in plants with a stronger stalk than the unedited control. Nucleotide diversity analysis revealed that the promoter of stiff1 was under strong selection in the maize stiff-stalk group. Our cloning of stiff1 reveals a case in which a transposable element played an important role in maize improvement. The identification of stiff1 and our edited stiff1 allele pave the way for efficient improvement of maize stalk strength.
中文翻译：
在硬度1启动子中插入大的转座子可增加玉米的茎强度。
茎倒伏通常由茎秆强度决定，会导致相当大的产量损失，并已成为高密度种植下玉米产量的主要威胁。然而，玉米茎秆强度的分子遗传基础仍不清楚，改良方法仍然低效。在这里，我们结合了基于图谱的克隆和关联作图，并鉴定了玉米茎强度主要数量性状基因座下的基因iffid1。 27.2 kb 转座元件插入存在于stiff1 基因的启动子中，该基因编码F-box 结构域蛋白。这种转座元件的插入抑制了stiff1的转录，导致细胞壁中的纤维素和木质素含量增加，从而提高了茎的强度。此外，通过 CRISPR/Cas9 系统生成的精确编辑的stiff1等位基因使植物的茎比未经编辑的对照更强。核苷酸多样性分析表明，在玉米硬秆组中，stiff1的启动子受到强选择。我们对stiff1的克隆揭示了转座元件在玉米改良中发挥重要作用的情况。硬度1和我们编辑的硬度1等位基因的鉴定为有效提高玉米茎秆强度铺平了道路。
更新日期：2020-01-11
中文翻译：
在硬度1启动子中插入大的转座子可增加玉米的茎强度。
茎倒伏通常由茎秆强度决定，会导致相当大的产量损失，并已成为高密度种植下玉米产量的主要威胁。然而，玉米茎秆强度的分子遗传基础仍不清楚，改良方法仍然低效。在这里，我们结合了基于图谱的克隆和关联作图，并鉴定了玉米茎强度主要数量性状基因座下的基因iffid1。 27.2 kb 转座元件插入存在于stiff1 基因的启动子中，该基因编码F-box 结构域蛋白。这种转座元件的插入抑制了stiff1的转录，导致细胞壁中的纤维素和木质素含量增加，从而提高了茎的强度。此外，通过 CRISPR/Cas9 系统生成的精确编辑的stiff1等位基因使植物的茎比未经编辑的对照更强。核苷酸多样性分析表明，在玉米硬秆组中，stiff1的启动子受到强选择。我们对stiff1的克隆揭示了转座元件在玉米改良中发挥重要作用的情况。硬度1和我们编辑的硬度1等位基因的鉴定为有效提高玉米茎秆强度铺平了道路。
京公网安备 11010802027423号