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Morphological and functional characteristics of aging kidneys based on two-photon microscopy in vivo.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2019-11-28 , DOI: 10.1002/jbio.201900246 Shulian Wu 1, 2 , Hengchang Guo 2 , Hannah Horng 2 , Yi Liu 2 , Hui Li 1 , Parnaz Daneshpajouhnejad 3 , Avi Rosenberg 3 , Christopher Albanese 4 , Suman Ranjit 4 , Peter M Andrews 4 , Moshe Levi 4 , Qinggong Tang 2, 5 , Yu Chen 1, 2
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2019-11-28 , DOI: 10.1002/jbio.201900246 Shulian Wu 1, 2 , Hengchang Guo 2 , Hannah Horng 2 , Yi Liu 2 , Hui Li 1 , Parnaz Daneshpajouhnejad 3 , Avi Rosenberg 3 , Christopher Albanese 4 , Suman Ranjit 4 , Peter M Andrews 4 , Moshe Levi 4 , Qinggong Tang 2, 5 , Yu Chen 1, 2
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Age‐related kidney disease, which is chronic and naturally occurring, is a general term for a set of heterogeneous disorders affecting kidney structures and characterized by a decline in renal function. Age‐related renal insufficiency has important implications with regard to body homeostasis, drug toxicity and renal transplantation. In our study, two‐photon microscopy was used to image kidney morphological and functional characteristics in an age‐related rat model in vivo. The changes in morphology are analyzed based on autofluorescence and Hoechst 33342 labeling in rats with different ages. Structural parameters including renal tubular diameter, cell nuclei density, size and shape are studied and compared with Hematoxylin and Eosin histological analysis. Functional characteristics, such as blood flow, and glomerular filtration rate are studied with high‐molecular weight (MW) 500‐kDa dextran‐fluorescein and low‐MW 10‐kDa dextran‐rhodamine. Results indicate that morphology changes significantly and functional characteristics deteriorate with age. These parameters are potential indicators for evaluating age‐related renal morphology and function changes. Combined analyses of these parameters could provide a quantitative, novel method for monitoring kidney diseases and/or therapeutic effects of kidney drugs.
中文翻译：
基于体内双光子显微镜的衰老肾脏的形态和功能特征。
年龄相关的肾脏疾病是一种慢性且自然发生的疾病，是一系列影响肾脏结构并以肾功能下降为特征的异质性疾病的总称。年龄相关的肾功能不全对身体的稳态，药物毒性和肾移植具有重要意义。在我们的研究中，使用双光子显微镜对体内与年龄相关的大鼠模型的肾脏形态和功能特征进行成像。基于自体荧光和Hoechst 33342标记分析不同年龄大鼠的形态学变化。研究了包括肾小管直径，细胞核密度，大小和形状在内的结构参数，并与苏木精和曙红组织学分析进行了比较。功能特征，例如血流，高分子量（MW）500-kDa葡聚糖-荧光素和低MW 10-kDa葡聚糖-罗丹明对肾小球滤过率和肾小球滤过率进行了研究。结果表明，随着年龄的增长，形态发生显着变化，功能特征恶化。这些参数是评估与年龄相关的肾脏形态和功能变化的潜在指标。这些参数的组合分析可以提供一种定量，新颖的方法来监测肾脏疾病和/或肾脏药物的治疗效果。
更新日期：2019-11-28
中文翻译：
基于体内双光子显微镜的衰老肾脏的形态和功能特征。
年龄相关的肾脏疾病是一种慢性且自然发生的疾病，是一系列影响肾脏结构并以肾功能下降为特征的异质性疾病的总称。年龄相关的肾功能不全对身体的稳态，药物毒性和肾移植具有重要意义。在我们的研究中，使用双光子显微镜对体内与年龄相关的大鼠模型的肾脏形态和功能特征进行成像。基于自体荧光和Hoechst 33342标记分析不同年龄大鼠的形态学变化。研究了包括肾小管直径，细胞核密度，大小和形状在内的结构参数，并与苏木精和曙红组织学分析进行了比较。功能特征，例如血流，高分子量（MW）500-kDa葡聚糖-荧光素和低MW 10-kDa葡聚糖-罗丹明对肾小球滤过率和肾小球滤过率进行了研究。结果表明，随着年龄的增长，形态发生显着变化，功能特征恶化。这些参数是评估与年龄相关的肾脏形态和功能变化的潜在指标。这些参数的组合分析可以提供一种定量，新颖的方法来监测肾脏疾病和/或肾脏药物的治疗效果。
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