The HOG (high-osmolarity glycerol) pathway is critical for the appropriate adaptation to adverse conditions. Here, we demonstrated that the deletion of CgHog1 resulted in enhanced sensitivity to osmotic stress and increased resistance to fludioxonil in the poplar anthracnose fungus Colletotrichum gloeosporioides. The accumulation of chitin around hyphal tips was obviously decreased in the ΔCgHog1 strain under sorbitol, whereas it strongly was increased in the response to fludioxonil compared with the wild type. To investigate the underlying mechanism of CgHog1-mediated adaption to osmotic stress and fludioxonil, transcriptomic profiles were performed in both the ΔCgHog1 strain and the wild type under the treatment of sorbitol and fludioxonil, respectively. Under sorbitol, genes associated with glycolysis, lipid metabolism, and accumulation of soluble sugars and amino acids were differentially expressed; under fludioxonil, vesicle trafficking-related genes were highly downregulated in the ΔCgHog1 strain, which was consistent with abnormal vacuoles distribution and morphology of hyphae, indicating that the growth defect caused by fludioxonil may be associated with disruption of endocytosis. Taken together, we elucidated the adaptation mechanisms of how CgHog1 regulates appropriate response to sorbitol and fludioxonil via different metabolism pathways. These findings extend our insights into the HOG pathway in fungi.

中文翻译：

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CgHog1控制在炭疽菌中对山梨糖醇和氟地西尼的适应性。

HOG（高渗甘油）途径对于适当适应不利条件至关重要。在这里，我们证明了CgHog1的缺失导致对杨树炭疽病真菌炭疽菌（Colletotrichum gloeosporioides）的渗透压力增强了敏感性，并增加了对氟狄克尼的抗性。在山梨糖醇条件下，ΔCgHog1菌株的甲壳质在菌丝尖端周围的积累明显减少，而对氟地莫尼的反应则比野生型显着增加了几丁质的积累。为了研究CgHog1介导的对渗透胁迫和氟地西尼适应性的潜在机制，分别在山梨糖醇和氟地西尼的处理下，在ΔCgHog1菌株和野生型中均进行了转录组学分析。在山梨醇的作用下，与糖酵解，脂质代谢，可溶性糖和氨基酸的积累差异表达；在氟地沙腈下，ΔCgHog1菌株中与囊泡运输相关的基因高度下调，这与液泡分布和菌丝的形态异常一致，表明氟地沙酮引起的生长缺陷可能与内吞作用的破坏有关。两者合计，我们阐明了CgHog1如何通过不同的代谢途径调节对山梨糖醇和氟地西尼的适当反应的适应机制。这些发现扩展了我们对真菌HOG途径的见解。提示由氟地西尼引起的生长缺陷可能与内吞作用的破坏有关。两者合计，我们阐明了CgHog1如何通过不同的代谢途径调节对山梨糖醇和氟地西尼的适当反应的适应机制。这些发现扩展了我们对真菌HOG途径的见解。提示由氟地西尼引起的生长缺陷可能与内吞作用的破坏有关。两者合计，我们阐明了CgHog1如何通过不同的代谢途径调节对山梨糖醇和氟地西尼的适当反应的适应机制。这些发现扩展了我们对真菌HOG途径的见解。