Abstract During the past two decades, cell culture has played an important role in many biochemical and biomedical fields. However, finding an alternative to animal serum is an important area in cell culture methods and related biotechnology industries. In a previous study, we demonstrated that the platelet-rich plasma (PRP) from specific pathogen-free pigs was a rich source of cytokines and growth factors and might serve as a replacement for animal serum. The selection of an activator is a crucial step in PRP activation and affects the availability of bioactive molecules. The present study is the first to demonstrate the ability of ectoine to activate PRP and to investigate the effects of ectoine-activated PRP on cell proliferation and biological function. The amounts of bioactive molecules, including TGF-β, PDGF-AB, EGF, VEGF, KGF and IGF, secreted from PRP activated by ectoine were measured. In addition, two types of primary cells and three types of cell lines were cultured with various concentrations of ectoine-activated PRP under animal serum-free conditions. Then, the effects of ectoine-activated PRP on cell growth kinetics, extracellular matrix secretion and melanin synthesis were also determined. Finally, our results demonstrated that ectoine is a novel and effective PRP activator and that ectoine-activated PRP can serve as an excellent replacement for animal serum in cell culture and related applications.

中文翻译：

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新型激活剂四氢嘧啶激活的无特定病原体猪富血小板血浆对细胞增殖和生物学功能的影响

摘要 在过去的二十年中，细胞培养在许多生化和生物医学领域发挥了重要作用。然而，寻找动物血清的替代品是细胞培养方法和相关生物技术行业的一个重要领域。在之前的一项研究中，我们证明了来自特定无病原体猪的富含血小板的血浆 (PRP) 是细胞因子和生长因子的丰富来源，可以作为动物血清的替代品。激活剂的选择是 PRP 激活的关键步骤，会影响生物活性分子的可用性。本研究首次证明了四氢嘧啶激活 PRP 的能力，并研究了四氢嘧啶激活的 PRP 对细胞增殖和生物学功能的影响。生物活性分子的数量，包括 TGF-β、PDGF-AB、EGF、VEGF、KGF 和 IGF，测量了由四氢嘧啶激活的 PRP 分泌。此外，在动物无血清条件下，用不同浓度的四氢嘧啶激活的 PRP 培养两种类型的原代细胞和三种类型的细胞系。然后，还确定了四氢嘧啶激活的 PRP 对细胞生长动力学、细胞外基质分泌和黑色素合成的影响。最后，我们的结果表明，四氢嘧啶是一种新型有效的 PRP 激活剂，四氢嘧啶激活的 PRP 可以作为细胞培养和相关应用中动物血清的绝佳替代品。还测定了四氢嘧啶激活的 PRP 对细胞生长动力学、细胞外基质分泌和黑色素合成的影响。最后，我们的结果表明，四氢嘧啶是一种新型有效的 PRP 激活剂，四氢嘧啶激活的 PRP 可以作为细胞培养和相关应用中动物血清的绝佳替代品。还确定了四氢嘧啶激活的 PRP 对细胞生长动力学、细胞外基质分泌和黑色素合成的影响。最后，我们的结果表明，四氢嘧啶是一种新型有效的 PRP 激活剂，四氢嘧啶激活的 PRP 可以作为细胞培养和相关应用中动物血清的绝佳替代品。