Abstract A quantitative analytical method to determine the dead cell ratio of bacteria (death ratio) in sludge was developed for evaluating technologies and determining a sufficient amount of ozone for the sludge ozone process. The method with SYTO9 and propidium iodide as staining agents for live and dead cells, respectively, was optimized. The death ratio developed in this study could successfully predict the reduction ratio of the excess sludge in the sludge ozone process with different ozone dosages. Consequently, the death ratio was proven to be a suitable indicator for excess sludge reduction.

中文翻译：

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污泥絮体中细菌的死细胞率作为污泥臭氧处理中污泥减量的指标

摘要 开发了一种定量分析方法来测定污泥中细菌的死细胞比（死亡比），用于评价技术和确定污泥臭氧过程中足够的臭氧量。对以SYTO9和碘化丙啶分别作为活细胞和死细胞染色剂的方法进行了优化。本研究开发的死亡率可以成功预测不同臭氧剂量下污泥臭氧过程中剩余污泥的减少率。因此，死亡率被证明是减少剩余污泥的合适指标。