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Immobilization of natural lipid biomembranes and their interactions with choline carboxylates. A nanoplasmonic sensing study.
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2019-11-05 , DOI: 10.1016/j.bbamem.2019.183115 Filip Duša 1 , Wen Chen 2 , Joanna Witos 3 , Antti H Rantamäki 2 , Alistair W T King 2 , Evangelos Sklavounos 4 , Michal Roth 1 , Susanne K Wiedmer 2
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2019-11-05 , DOI: 10.1016/j.bbamem.2019.183115 Filip Duša 1 , Wen Chen 2 , Joanna Witos 3 , Antti H Rantamäki 2 , Alistair W T King 2 , Evangelos Sklavounos 4 , Michal Roth 1 , Susanne K Wiedmer 2
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The cell membrane is mainly composed of lipid bilayers with inserted proteins and carbohydrates. Lipid bilayers made of purified or synthetic lipids are widely used for estimating the effect of target compounds on cell membranes. However, the composition of such biomimetic membranes is much simpler than the composition of biological membranes. Interactions between compounds and simple composition biomimetic membranes might not demonstrate the effect of target compounds as precisely as membranes with compositions close to real organisms. Therefore, the aim of our study is to construct biomimetic membrane closely mimicking the state of natural membranes. Liposomes were prepared from lipids extracted from L-α-phosphatidylcholine, Escherichia coli, yeast (Saccharomyces cerevisiae) and bovine liver cells through agitation and sonication. They were immobilized onto silicon dioxide (SiO2) sensor surfaces using N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid buffer with calcium chloride. The biomimetic membranes were successfully immobilized onto the SiO2 sensor surface and detected by nanoplasmonic sensing. The immobilized membranes were exposed to choline carboxylates. The membrane disruption effect was, as expected, more pronounced with increasing carbohydrate chain length of the carboxylates. The results correlated with the toxicity values determined using Vibrio fischeri bacteria. The yeast extracted lipid membranes had the strongest response to introduction of choline laurate while the bovine liver lipid extracted liposomes were the most sensitive towards the shorter choline carboxylates. This implies that the composition of the cell membrane plays a crucial role upon interaction with choline carboxylates, and underlines the necessity of testing membrane systems of different origin to obtain an overall image of such interactions.
中文翻译：
天然脂质生物膜的固定化及其与胆碱羧酸盐的相互作用。纳米等离子体传感研究。
细胞膜主要由脂质双层以及插入的蛋白质和碳水化合物组成。由纯化或合成脂质制成的脂质双层被广泛用于评估目标化合物对细胞膜的作用。但是，这种仿生膜的组成比生物膜的组成简单得多。化合物与简单组成的仿生膜之间的相互作用可能无法像组成接近真实生物的膜那样精确地证明目标化合物的效果。因此，我们研究的目的是构建与天然膜状态非常相似的仿生膜。通过搅动和超声处理，从L-α-磷脂酰胆碱，大肠杆菌，酵母（酿酒酵母）和牛肝细胞提取的脂质中制备脂质体。使用带有氯化钙的N-（2-羟乙基）哌嗪-N'-2-乙烷磺酸缓冲液将它们固定在二氧化硅（SiO2）传感器表面上。仿生膜已成功固定在SiO2传感器表面，并通过纳米等离子体传感进行检测。使固定的膜暴露于胆碱羧酸盐。如所预期的，随着羧酸盐的碳水化合物链长度的增加，膜破坏作用更加明显。结果与使用费氏弧菌细菌测定的毒性值相关。酵母提取的脂质膜对月桂酸胆碱的引入反应最强，而牛肝脂质提取的脂质体对较短的胆碱羧酸盐最敏感。
更新日期：2019-11-05
中文翻译：
天然脂质生物膜的固定化及其与胆碱羧酸盐的相互作用。纳米等离子体传感研究。
细胞膜主要由脂质双层以及插入的蛋白质和碳水化合物组成。由纯化或合成脂质制成的脂质双层被广泛用于评估目标化合物对细胞膜的作用。但是，这种仿生膜的组成比生物膜的组成简单得多。化合物与简单组成的仿生膜之间的相互作用可能无法像组成接近真实生物的膜那样精确地证明目标化合物的效果。因此，我们研究的目的是构建与天然膜状态非常相似的仿生膜。通过搅动和超声处理，从L-α-磷脂酰胆碱，大肠杆菌，酵母（酿酒酵母）和牛肝细胞提取的脂质中制备脂质体。使用带有氯化钙的N-（2-羟乙基）哌嗪-N'-2-乙烷磺酸缓冲液将它们固定在二氧化硅（SiO2）传感器表面上。仿生膜已成功固定在SiO2传感器表面，并通过纳米等离子体传感进行检测。使固定的膜暴露于胆碱羧酸盐。如所预期的，随着羧酸盐的碳水化合物链长度的增加，膜破坏作用更加明显。结果与使用费氏弧菌细菌测定的毒性值相关。酵母提取的脂质膜对月桂酸胆碱的引入反应最强，而牛肝脂质提取的脂质体对较短的胆碱羧酸盐最敏感。
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