Promoting highly unsaturated fatty acid (HUFA) uptake and deposition can improve nutritional value of farmed fish and reduce dietary fish oil addition. Previously, we found that the golden pompano Trachinotus ovatus liver HUFA content increased with the increasing of dietary HUFA. Therefore, we examined the common genes and pathways responsible for HUFA uptake and deposition in T. ovatus liver using transcriptome sequencing technology after feeding with either 1.0% or 2.1% HUFA for 8 weeks. Results showed that a total of 140 and 147 genes were significantly upregulated and downregulated, respectively. Five bile acid synthesis-related genes (CYP7A1, CYP8B1, AKR1D1, SCP2 and ACOT8), which are related to dietary fat emulsification were downregulated in 2.1% HUFA group, implying that the cholate synthesized through the classical pathway might be the main bile acid form in fat emulsification. Moreover, fatty acid transport protein (FATP)-6, fatty acid binding protein (FABP)-1, -4, and -6 increased with HUFA deposition, especially FATP6 and FABP4, suggesting that the two genes may be important mediators involved in HUFA uptake and deposition. KEGG analysis showed that most of the differential genes described above were involved in peroxisome proliferator activator receptor (PPAR) signaling pathway, and PPARγ increased with HUFA deposition, indicating that PPARγ might be a key regulator of HUFA uptake and deposition by regulating the genes involved in fatty acid emulsification and transport. This study focused on the liver, which is the center of intermediary metabolism, providing a comprehensive understanding of the molecular regulation of HUFA uptake and deposition in T. ovatus, which should be further investigated to develop potential measures to improve HUFA content.

中文翻译：

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金色mp鱼（Trachinotus ovatus）肝脏的转录组分析表明，潜在的调控靶标涉及HUFA摄取和沉积。

促进高度不饱和脂肪酸（HUFA）的吸收和沉积可以提高养殖鱼类的营养价值，并减少膳食鱼油的添加。以前，我们发现随着饮食中HUFA含量的增加，金色mp鱼Trachinotus ovatus肝脏HUFA含量也随之增加。因此，在喂食1.0％或2.1％HUFA的8周后，我们使用转录组测序技术研究了卵圆线虫肝脏中HUFA摄取和沉积的常见基因和途径。结果表明，总共有140和147个基因分别显着上调和下调。2.1％HUFA组下调了与饮食脂肪乳化相关的五个与胆汁酸合成相关的基因（CYP7A1，CYP8B1，AKR1D1，SCP2和ACOT8），暗示通过经典途径合成的胆酸盐可能是脂肪乳化中的主要胆汁酸形式。此外，脂肪酸转运蛋白（FATP）-6，脂肪酸结合蛋白（FABP）-1，-4和-6随着HUFA的沉积而增加，尤其是FATP6和FABP4，表明这两个基因可能是参与HUFA的重要介体。吸收和沉积。KEGG分析表明，上述大多数差异基因均参与过氧化物酶体增殖物激活剂受体（PPAR）信号传导途径，并且PPARγ随着HUFA沉积而增加，表明PPARγ可能是通过调节参与该基因的HUFA摄取和沉积的关键调节剂。脂肪酸的乳化和运输。这项研究的重点是肝脏，它是中间代谢的中心，