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Chronic exposure to venlafaxine and increased water temperature reversibly alters microRNA in zebrafish gonads (Danio rerio).
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2019-11-04 , DOI: 10.1016/j.cbd.2019.100634 Heather Ikert 1 , Paul M Craig 1
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2019-11-04 , DOI: 10.1016/j.cbd.2019.100634 Heather Ikert 1 , Paul M Craig 1
Affiliation
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MicroRNA (miRNA) are short, non-coding RNA that act by downregulating targeted mRNA transcripts. Only recently have they been used as endpoints in studies of aquatic toxicology. The purpose of this study was to determine the effect of an antidepressant contaminant, venlafaxine (VFX), and increased temperature on specific microRNA levels in zebrafish (Danio rerio) reproductive tissue. Adult zebrafish were exposed to one of four conditions; control, 1 μg/L VFX (VFX), 32 °C (Temp), or 1 μg/L VFX + 32 °C (VFX & Temp) for 21 days. Half of the fish were returned to control conditions for a 21-day recovery period. RT-qPCR was performed to measure relative abundances of several miRNAs known to respond to antidepressant exposure: dre-miR-22b-3p, dre-miR-301a, dre-miR-140-5p, dre-let-7d-5p, dre-miR-210-5p, and dre-miR-457b-5p. After the exposure period, dre-miR-22b-3p and dre-miR-301a showed a significant downregulation in response to all treatments. In contrast, after the recovery period, there were no significant differences in microRNA abundance. These altered microRNA are predicted to target several genes, including phosphofructokinase, and are associated with ovarian pathologies. Combined, we have shown that VFX and increased water temperature alter miRNA abundances in zebrafish reproductive tissue, an effect correlated with a functional stress response and cell cycle dysregulation.
中文翻译：
长期暴露于文拉法辛和水温升高可逆地改变斑马鱼性腺（Danio rerio）中的microRNA。
MicroRNA（miRNA）是短的非编码RNA，可通过下调靶向的mRNA转录物来发挥作用。直到最近，它们才被用作水生毒理学研究的终点。这项研究的目的是确定抗抑郁剂文拉法辛（VFX）和温度升高对斑马鱼（Danio rerio）生殖组织中特定microRNA水平的影响。成年斑马鱼暴露于以下四种条件之一：对照，1μg/ L VFX（VFX），32°C（温度）或1μg/ L VFX + 32°C（VFX和温度）21天。一半的鱼恢复了控制条件，恢复了21天。进行RT-qPCR以测量已知对抗抑郁剂暴露有反应的几种miRNA的相对丰度：dre-miR-22b-3p，dre-miR-301a，dre-miR-140-5p，dre-let-7d-5p，dre -miR-210-5p和dre-miR-457b-5p。暴露期过后，dre-miR-22b-3p和dre-miR-301a对所有处理均显示出明显的下调。相反，在恢复期之后，microRNA丰度没有显着差异。预测这些改变的microRNA靶向几种基因，包括果糖磷酸激酶，并与卵巢病理相关。结合起来，我们已经显示，VFX和升高的水温改变了斑马鱼生殖组织中的miRNA丰度，这种作用与功能性应激反应和细胞周期失调有关。并与卵巢病变有关。结合起来，我们已经显示，VFX和升高的水温改变了斑马鱼生殖组织中的miRNA丰度，这种作用与功能性应激反应和细胞周期失调有关。并与卵巢病变有关。结合起来，我们已经显示，VFX和升高的水温改变了斑马鱼生殖组织中的miRNA丰度，这种作用与功能性应激反应和细胞周期失调有关。
更新日期：2019-11-04
中文翻译：
长期暴露于文拉法辛和水温升高可逆地改变斑马鱼性腺（Danio rerio）中的microRNA。
MicroRNA（miRNA）是短的非编码RNA，可通过下调靶向的mRNA转录物来发挥作用。直到最近，它们才被用作水生毒理学研究的终点。这项研究的目的是确定抗抑郁剂文拉法辛（VFX）和温度升高对斑马鱼（Danio rerio）生殖组织中特定microRNA水平的影响。成年斑马鱼暴露于以下四种条件之一：对照，1μg/ L VFX（VFX），32°C（温度）或1μg/ L VFX + 32°C（VFX和温度）21天。一半的鱼恢复了控制条件，恢复了21天。进行RT-qPCR以测量已知对抗抑郁剂暴露有反应的几种miRNA的相对丰度：dre-miR-22b-3p，dre-miR-301a，dre-miR-140-5p，dre-let-7d-5p，dre -miR-210-5p和dre-miR-457b-5p。暴露期过后，dre-miR-22b-3p和dre-miR-301a对所有处理均显示出明显的下调。相反，在恢复期之后，microRNA丰度没有显着差异。预测这些改变的microRNA靶向几种基因，包括果糖磷酸激酶，并与卵巢病理相关。结合起来，我们已经显示，VFX和升高的水温改变了斑马鱼生殖组织中的miRNA丰度，这种作用与功能性应激反应和细胞周期失调有关。并与卵巢病变有关。结合起来，我们已经显示，VFX和升高的水温改变了斑马鱼生殖组织中的miRNA丰度，这种作用与功能性应激反应和细胞周期失调有关。并与卵巢病变有关。结合起来，我们已经显示，VFX和升高的水温改变了斑马鱼生殖组织中的miRNA丰度，这种作用与功能性应激反应和细胞周期失调有关。
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