Genetic basis for the cooperative bioactivation of plant lignans by Eggerthella lenta and other human gut bacteria.,Nature Microbiology

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Genetic basis for the cooperative bioactivation of plant lignans by Eggerthella lenta and other human gut bacteria.
Nature Microbiology ( IF 20.5 ) Pub Date : 2019-11-04 , DOI: 10.1038/s41564-019-0596-1
Elizabeth N Bess _{1,

2,

3} , Jordan E Bisanz ₁ , Fauna Yarza ₁ , Annamarie Bustion ₁ , Barry E Rich ₂ , Xingnan Li ₄ , Seiya Kitamura ₅ , Emily Waligurski ₁ , Qi Yan Ang ₁ , Diana L Alba ₆ , Peter Spanogiannopoulos ₁ , Stephen Nayfach ₇ , Suneil K Koliwad ₆ , Dennis W Wolan ₅ , Adrian A Franke ₄ , Peter J Turnbaugh _{1,

8}

Affiliation

Plant-derived lignans, consumed daily by most individuals, are thought to protect against cancer and other diseases1; however, their bioactivity requires gut bacterial conversion to enterolignans2. Here, we dissect a four-species bacterial consortium sufficient for all five reactions in this pathway. A single enzyme (benzyl ether reductase, encoded by the gene ber) was sufficient for the first two biotransformations, variable between strains of Eggerthella lenta, critical for enterolignan production in gnotobiotic mice and unique to Coriobacteriia. Transcriptional profiling (RNA sequencing) independently identified ber and genomic loci upregulated by each of the remaining substrates. Despite their low abundance in gut microbiomes and restricted phylogenetic range, all of the identified genes were detectable in the distal gut microbiomes of most individuals living in northern California. Together, these results emphasize the importance of considering strain-level variations and bacterial co-occurrence to gain a mechanistic understanding of the bioactivation of plant secondary metabolites by the human gut microbiome.

中文翻译：

Eggerthella lenta 和其他人类肠道细菌协同生物激活植物木脂素的遗传基础。

大多数人每天食用的源自植物的木脂素被认为可以预防癌症和其他疾病1；然而，它们的生物活性需要肠道细菌转化为肠木脂素2。在这里，我们剖析了一个足以满足该途径中所有五种反应的四种细菌联合体。一种酶（苄基醚还原酶，由基因 ber 编码）足以用于前两次生物转化，在长效卵菌菌株之间发生变化，这对无菌小鼠的肠木脂素生产至关重要，并且是 Coriobacteriia 独有的。转录谱分析（RNA 测序）独立地鉴定了被其余底物上调的 ber 和基因组位点。尽管它们在肠道微生物组中的丰度较低且系统发育范围有限，在加利福尼亚北部的大多数人的远端肠道微生物组中，所有已鉴定的基因都可以检测到。总之，这些结果强调了考虑菌株水平变化和细菌共存的重要性，以获得对人体肠道微生物组对植物次生代谢物的生物活化的机械理解。

更新日期：2019-11-04

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