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β-Arrestin 1 (ARRB1) serves as a molecular marker of the peripheral circadian rhythm.
International Journal of Oral Science ( IF 10.8 ) Pub Date : 2019-11-05 , DOI: 10.1038/s41368-019-0065-y
Tatsunosuke Tomita 1 , Taisuke Mori 2 , Yoshiaki Onishi 1
Affiliation  

The control of the circadian rhythm is important for health because it regulates physiological functions and is associated with health hazards. We aimed to identify a circadian biomarker of health status in human saliva, since collecting saliva is non-invasive, straightforward, and cost-effective. Among 500 genes potentially controlled by the salivary clock identified using chromatin immunoprecipitation (ChIP) assays, 22 of them showed reasonable transcriptional responses according to a DNA array in a salivary model system. Among these 22 genes, ARRB1, which is expressed in human salivary glands, was also expressed in model HSG cells at the transcriptional and translational levels. The profile of ARRB1 expression in human saliva was circadian, suggesting that ARRB1 could serve as a candidate circadian biomarker in saliva. We compared ARRB1 with other biomarkers in salivary samples from jet-lagged individuals. The circadian profile of ARRB1 reflected the time lag more than the profile of melatonin, whereas the profiles of cortisol and α-amylase did not reflect the time lag. Overall, these findings suggest that salivary ARRB1 could serve as a candidate biomarker that could be used to monitor the internal body clock.

中文翻译:

β-arrestin1(ARRB1)充当周围昼夜节律的分子标记。

昼夜节律的控制对健康很重要,因为它调节生理功能并与健康危害相关。我们的目的是确定人类唾液中健康状况的昼夜节律生物标志物,因为收集唾液是非侵入性,直接且具有成本效益的。在500个可能受唾液钟控制的基因中,染色质免疫沉淀(ChIP)分析确定了其中的22个基因,这些基因根据唾液模型系统中的DNA阵列显示出合理的转录反应。在这22个基因中,在人类唾液腺中表达的ARRB1在模型HSG细胞中也以转录和翻译水平表达。人唾液中ARRB1表达的概况是昼夜节律的,表明ARRB1可以作为唾液中候选的昼夜节律生物标志物。我们将ARRB1与其他生物标记物从时差低下的人的唾液样本中进行了比较。ARRB1的昼夜节律特征比褪黑素特征更能反映出时滞,而皮质醇和α-淀粉酶的特征却不能反映出时滞。总体而言,这些发现表明唾液中的ARRB1可以作为候选生物标志物,用于监测体内生物钟。
更新日期:2019-11-05
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