当前位置: X-MOL 学术Clin. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Estimating short- and long-term reference change values and index of individuality for tests of platelet function.
Clinical Biochemistry ( IF 2.5 ) Pub Date : 2019-11-04 , DOI: 10.1016/j.clinbiochem.2019.10.001
Brooke M Katzman 1 , Amy M Wockenfus 1 , Renee J Scott 1 , Sandra C Bryant 2 , Allan S Jaffe 3 , Brad S Karon 1
Affiliation  

BACKGROUND In order to manage risks of bleeding and thrombosis after some surgical procedures, platelet function is often measured repeatedly over days or weeks using laboratory tests of platelet function. To interpret test results in the perioperative period, it is necessary to understand analytical, biological and between-person variation. METHODS We collected three separate blood specimens from 16 healthy volunteers on the first study day, and one additional specimen from each volunteer 1, 2, and 3 months later. Arachidonic acid-induced and adenosine diphosphate (ADP)-induced platelet function were measured in duplicate by whole blood impedance aggregometry using Multiplate (ASPI/ADP tests) and VerifyNow (Aspirin Reaction Units [ARU] and P2Y12 Reaction Units [PRU]). The analytical variation (CVA), within-subject variation (CVI), between-subject variation (CVG), index of individuality (II), and reference change values (RCV) were calculated. RESULTS VerifyNow ARU demonstrated the smallest short-term and long-term variability (CVA, CVI, and CVG ~1%), resulting in short- and long-term RCV values <5%. II was also higher (1.92) for VerifyNow ARU than other platelet function tests. Multiplate ASPI and ADP tests had the highest RCV both short-(19.0% and 25.2%, respectively) and long-term (32.1% and 39.6%, respectively) due to increased CVA (>5%) and CVI (3.9-13.1%). VerifyNow PRU had a lower RCV than Multiplate ADP; but was the only test with II <0.6. CONCLUSIONS VerifyNow ARU results can be interpreted relative to a fixed cut-off or population-based reference interval; or relative to small changes in an individual's previous values. VerifyNow PRU and Multiplate ASPI and ADP tests should only be interpreted based upon relative change; and can only distinguish relatively large (>23%) changes over several weeks.

中文翻译:

估算短期和长期参考变化值以及用于血小板功能测试的个性指数。

背景技术为了处理一些外科手术后的出血和血栓形成的风险,经常使用血小板功能的实验室测试在几天或几周内反复测量血小板功能。为了解释围手术期的测试结果,有必要了解分析,生物学和人与人之间的差异。方法在研究的第一天,我们从16名健康志愿者那里采集了三份单独的血液样本,然后在1、2和3个月后从每位志愿者那里收集了另外一份样本。使用Multiplate(ASPI / ADP测试)和VerifyNow(阿司匹林反应单位[ARU]和P2Y12反应单位[PRU]),通过全血阻抗聚集法一式两份测量花生四烯酸诱导的和腺苷二磷酸(ADP)诱导的血小板功能。分析变异(CVA),受试者内部变异(CVI),计算受试者之间的差异(CVG),个性指数(II)和参考变化值(RCV)。结果VerifyNow ARU表现出最小的短期和长期变异性(CVA,CVI和CVG〜1%),导致短期和长期RCV值<5%。VerifyNow ARU的II也比其他血小板功能测试高(1.92)。由于CVA(> 5%)和CVI(3.9-13.1%)的增加,多板ASPI和ADP测试的短期RCV最高(分别为短期(分别为19.0%和25.2%)和长期(分别为32.1%和39.6%))。 )。VerifyNow PRU的RCV低于Multiplate ADP;但是是II <0.6的唯一测试。结论VerifyNow ARU结果可以相对于固定的临界值或基于人群的参考区间进​​行解释。或相对于个人先前值的微小变化。VerifyNow PRU和Multiplate ASPI和ADP测试仅应根据相对变化来解释。并且只能在几周内分辨出较大的变化(> 23%)。
更新日期:2019-11-04
down
wechat
bug