Inflammation and oxidative stress occurs in muscle of Duchenne muscular dystrophy (DMD). The relationship between a panel of biomarkers and the DMD outcome is necessary to indicate of disease progression and response to rehabilitation programs. The aim was to analyze the connective tissue of muscle of Mdx mice and immunoexpression of MMP-2, MMP-9, and 8-OHdG, which signalizes oxidative stress related to DNA damage. Biceps brachii of male C57BL/10 and C57BL/10-Dmdmdx mice was submitted to Hematoxylin-Eosin, Sirius red and immunohistochemistry (MMP-2, MMP-9 and 8-OHdG) analysis. Mdx showed focal lesions with intense inflammation and fibrosis related to immunoexpression of MMP-2 and MMP-9, proving the hypothesis that these MMPs are linked to muscular tissue degeneration, which can be regenerated by their inhibition, improving the treatment of DMD carriers. Histopathological findings related to centralized nuclei increase were related to higher 8-OHdG immunomarked nuclei in Mdx, which signalizes oxidative stress associated with DNA damage provoked by DMD. Such result shows that the evaluation of 8-OHdG during the evolution of the disease could be a method to evaluate DMD disease progression.

中文翻译：

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Mdx小鼠骨骼肌纤维化过程中金属蛋白酶2和9和8-OHdG的存在。

炎症和氧化应激发生在Duchenne肌营养不良症（DMD）的肌肉中。生物标志物组与DMD结果之间的关系对于指示疾病的进展和对康复计划的反应是必要的。目的是分析Mdx小鼠肌肉的结缔组织以及MMP-2，MMP-9和8-OHdG的免疫表达，这表明与DNA损伤有关的氧化应激。将雄性C57BL / 10和C57BL / 10-Dmdmdx小鼠的肱二头肌接受苏木精-曙红，天狼星红和免疫组织化学（MMP-2，MMP-9和8-OHdG）分析。Mdx显示局灶性病变具有与MMP-2和MMP-9的免疫表达有关的强烈炎症和纤维化，证明了这些MMP与肌肉组织变性有关，可以通过抑制它们而再生，改善DMD携带者的治疗。与中央核增加相关的组织病理学发现与Mdx中较高的8-OHdG免疫标记核相关，这表明与DMD引起的DNA损伤相关的氧化应激。这样的结果表明，在疾病发展过程中对8-OHdG的评估可能是评估DMD疾病进展的一种方法。