当前位置: X-MOL 学术J. Biophotonics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Optical coherence tomography as a noninvasive 3D real time imaging tool for the rapid evaluation of phenotypic variations in insect embryonic development.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2019-11-27 , DOI: 10.1002/jbio.201960047
Ya Su 1 , Liya Wei 2 , Hao Tan 1 , Jing Li 2 , Wenping Li 1 , Lei Fu 1 , Tongxin Wang 2 , Le Kang 2 , X Steve Yao 1
Affiliation  

Noninvasive visualization of embryos at different development stages is crucial for the understanding of the basic developmental biology. It is therefore desirable to have an imaging tool capable of rapidly evaluating the effects of gene manipulation or genome editing in developing embryos for the studies of gene functions and genetic engineering. Here, we propose and demonstrate a novel use of optical coherence tomography (OCT) to noninvasively exam the embryonic development of the migratory locusts in real time with 3‐dimensional (3D) view capability. In particular, we obtain the sufficiently high spatial resolution tomographic 2D and 3D images of live locust embryos throughout their development processes. We show that not only we are able to noninvasively observe all previously known forms of blastokinesis as an embryo develops, such as anatrepsis, katatrepsis, revolution, rotation and diapauses, and determine their precise occurring time or duration, but also discover an unreported rotation form we named “twist.” In addition, with the OCT images we determined the exact occurring time of diapauses of the locusts from Tibetan plateau for the first time. Finally, we demonstrate that OCT systems can be used to rapidly capture the development defects of genetically modified embryos in which certain genes essential for embryonic development were suppressed by RNA interference. Our work shows that OCT is an enabling imaging tool with sufficient spatial resolution for the rapid evaluation of embryonic variations of small animals.image

中文翻译:

光学相干断层扫描是一种快速评估昆虫胚胎发育中表型变异的无创3D实时成像工具。

胚胎在不同发育阶段的无创可视化对于理解基础发育生物学至关重要。因此,需要一种能够快速评估发育中的胚胎中的基因操纵或基因组编辑作用的成像工具,用于研究基因功能和基因工程。在这里,我们提出并演示了一种光学相干断层扫描(OCT)的新颖用法,它具有3D(3D)观察功能,可以实时无创地检查蝗虫的胚胎发育。尤其是,我们在整个蝗虫发育过程中获得了足够高的空间分辨率断层摄影2D和3D断层图像。我们证明,随着胚胎的发展,我们不仅能够无创地观察到所有以前已知的胚泡动力学形式,(例如尖锐度,角锥虫,旋转,旋转和滞育),并确定其确切的发生时间或持续时间,还发现了一种未报告的旋转形式,我们称之为“扭曲”。此外,利用OCT图像,我们首次确定了青藏高原蝗虫滞育的确切发生时间。最后,我们证明OCT系统可用于快速捕获转基因胚胎的发育缺陷,其中某些胚胎发育必不可少的基因被RNA干扰抑制。我们的工作表明,OCT是一种启用的成像工具,具有足够的空间分辨率,可以快速评估小型动物的胚胎变异。而且还发现了一个未报告的旋转形式,我们称为“扭曲”。此外,利用OCT图像,我们首次确定了青藏高原蝗虫滞育的确切发生时间。最后,我们证明OCT系统可用于快速捕获转基因胚胎的发育缺陷,其中某些胚胎发育必不可少的基因被RNA干扰抑制。我们的工作表明,OCT是一种启用的成像工具,具有足够的空间分辨率,可以快速评估小型动物的胚胎变异。而且还发现了一个未报告的旋转形式,我们称为“扭曲”。此外,利用OCT图像,我们首次确定了青藏高原蝗虫滞育的确切发生时间。最后,我们证明OCT系统可用于快速捕获转基因胚胎的发育缺陷,其中某些胚胎发育必不可少的基因被RNA干扰抑制。我们的工作表明,OCT是一种启用的成像工具,具有足够的空间分辨率,可以快速评估小型动物的胚胎变异。我们证明,OCT系统可用于快速捕获转基因胚胎的发育缺陷,其中某些胚胎发育必不可少的基因受到RNA干扰的抑制。我们的工作表明,OCT是一种启用的成像工具,具有足够的空间分辨率,可以快速评估小型动物的胚胎变异。我们证明,OCT系统可用于快速捕获转基因胚胎的发育缺陷,其中某些胚胎发育必不可少的基因受到RNA干扰的抑制。我们的工作表明,OCT是一种启用的成像工具,具有足够的空间分辨率,可以快速评估小型动物的胚胎变异。图像
更新日期:2019-11-27
down
wechat
bug