An autoantibody against a 48-Kd fragment of human DNA-topoiomerase I in breast cancer: Implication for diagnosis and prognosis, and antibody-dependent cellular cytotoxicity in vitro.,Cellular Immunology

当前位置： X-MOL 学术 › Cell. Immunol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

An autoantibody against a 48-Kd fragment of human DNA-topoiomerase I in breast cancer: Implication for diagnosis and prognosis, and antibody-dependent cellular cytotoxicity in vitro.
Cellular Immunology ( IF 3.7 ) Pub Date : 2019-11-02 , DOI: 10.1016/j.cellimm.2019.104007
Xu He ₁ , Xiao-Hui Jiang ₂ , Kevin Yi-Xiao Yie ₃ , Jie Chen ₁ , Jian-Bo Zhang ₁ , Shang-Mian Yie ₃

Affiliation

Previously, we reported a novel tumor-associated antigen (TAA) derived from human DNA-topoiomerase I (TOP 1). In the present study, we demonstrated that the autoantibody against the TAA could be a potential biomarker in the early diagnosis and favorable prognosis of patients with breast cancer (BC). To understand the survival benefits in BC patients, we investigated whether the autoantibody could induce antibody-dependent cellular cytotoxicity activities (ADCC) against breast cancer cells in vitro. We found that the autoantibody exhibited significant ADCC activities that destroyed breast cancer MCF-7 and MDA-MB-231cells with peripheral blood mononuclear cells (PBMCs). The ADCC activities of the autoantibody were significantly correlated with the number of natural killer (NK) cells, NKT cells, and CD4+/CD8+ T cells. Accordingly, our findings showed that the autoantibody not only represented an early index of immune response to the TAA, but also was involved in host immune defense mechanisms that initiated the destruction of cancer cells.

中文翻译：

针对乳腺癌中人类DNA拓扑异构酶I 48 Kd片段的自身抗体：对诊断和预后的影响以及体外抗体依赖性细胞的细胞毒性。

以前，我们报道了一种源自人类DNA拓扑异构酶I（TOP 1）的新型肿瘤相关抗原（TAA）。在本研究中，我们证明了针对TAA的自身抗体可能是乳腺癌（BC）患者早期诊断和良好预后的潜在生物标志物。为了了解BC患者的生存益处，我们研究了自身抗体是否可以在体外诱导针对乳腺癌细胞的抗体依赖性细胞毒性作用（ADCC）。我们发现自身抗体表现出显着的ADCC活性，该活性破坏了带有外周血单核细胞（PBMC）的乳腺癌MCF-7和MDA-MB-231细胞。自身抗体的ADCC活性与自然杀伤（NK）细胞，NKT细胞和CD4 + / CD8 + T细胞的数量显着相关。因此，

更新日期：2019-11-04

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11