Dermacentor silvarum is an important vector of tick-borne pathogens. The microbiota of ticks has been recognized to influence their development, fitness, and reproduction as well as the acquisition, establishment and transmission of pathogens. Eggs, larvae, nymphs and adults (females and males) of D. silvarum were used in this study to evaluate microbial community and diversity across different developmental stages. The results demonstrated that some developmental stages host different bacterial species, confirming that each stage of development could have a specific associated microbiota. Proteobacteria was the dominant phylum in most stages, while Actinobacteria was the most abundant in nymphs. The abundance of Brevibacterium significantly increased from the egg stage to the following stages, and there was a sharp increase in the abundance of this genus among nymphs. Eggs showed the highest microbial richness and diversity, and the microbial community of eggs exhibited high similarity to that of females. Moreover, the high abundance of Coxiella in eggs and females further indicated that members of this genus are vertically transmitted symbionts. Nymphs showed a microbial composition distinct from those of the other stages, and the microbial community associated with males possessed higher species richness and greater bacterial diversity than that of females. Further investigations are needed to determine the functions of the microbiota and provide information enabling a better understanding of the diversity of the microbial community at different life stages.

Silvarum Dermacentor silvarum是tick传播病原体的重要载体。壁虱的微生物群已被认为会影响其发育，适应性和繁殖以及病原体的获取，建立和传播。本研究使用了D. silvarum的卵，幼虫，若虫和成虫（雌雄）来评估不同发育阶段的微生物群落和多样性。结果表明，某些发育阶段具有不同的细菌种类，从而证实了每个发育阶段都可能具有特定的相关微生物群。在大多数阶段，变形杆菌是显性门，而若虫则是放线菌，是最丰富的门。短杆菌属的丰富性从卵期到随后的阶段显着增加，若虫中该属的丰度急剧增加。鸡蛋显示出最高的微生物丰富性和多样性，并且鸡蛋的微生物群落与雌性具有高度相似性。而且，Coxiella的丰度很高卵中的雌性和雌性进一步表明，该属的成员是垂直传播的共生体。若虫显示出不同于其他阶段的微生物组成，与雄性相关的微生物群落比雌性具有更高的物种丰富度和更大的细菌多样性。需要进行进一步的调查以确定微生物群的功能，并提供信息，使人们能够更好地了解不同生命阶段中微生物群落的多样性。