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Mycorrhizal symbiosis induces divergent patterns of transport and partitioning of Cd and Zn in Populus trichocarpa
Environmental and Experimental Botany ( IF 4.5 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.envexpbot.2019.103925
Vinicius Henrique De Oliveira , Ihsan Ullah , Jim M. Dunwell , Mark Tibbett

Abstract We investigated how arbuscular mycorrhizal symbiosis can alter trace element uptake, distribution and toxicity in plants by examining some of the molecular mechanisms behind Populus trichocarpa tolerance to Cd and Zn, and the effects of AMF in metal homeostasis. Plants were grown under Cd and Zn contamination, with and without Rhizophagus irregularis inoculation. We determined organ metal concentrations, the expression of genes involved in trace element homeostasis, and the function of metallothionein PtMT2b by heterologous expression in yeast. P. trichocarpa was highly tolerant to both elements, with AMF increasing Zn accumulation. AMF altered the partitioning of Cd, but maintained the same patterns for Zn, indicating that despite being geochemically similar and carried mostly by the same transporters, the nutrient metal (Zn) is handled differently from the non-essential metal (Cd). High Zn and Cd down-regulated PtHMA4 (roots), and up-regulated PtZIP1 (leaves), suggesting their involvement in transporting both metals in poplar. PtMT2b was highly up-regulated in mycorrhizal roots and enhanced Cd tolerance in transformed yeast. R. irregularis reduced Cd transfer to poplar shoots, but did not affect Zn partitioning. The gene expression patterns observed offer a glimpse into the mechanisms behind trace element uptake/translocation dynamic in poplars, influenced by AMF symbiosis.

中文翻译:

菌根共生诱导毛果杨中 Cd 和 Zn 运输和分配的不同模式

摘要 我们通过研究毛果杨对 Cd 和 Zn 耐受的一些分子机制以及 AMF 对金属稳态的影响,研究了丛枝菌根共生如何改变植物中微量元素的吸收、分布和毒性。植物在 Cd 和 Zn 污染下生长,接种和不接种不规则根瘤菌。我们通过酵母中的异源表达确定了器官金属浓度、参与微量元素稳态的基因的表达以及金属硫蛋白 PtMT2b 的功能。P. trichocarpa 对这两种元素都具有高度耐受性,AMF 会增加锌的积累。AMF 改变了 Cd 的分配,但对 Zn 保持相同的模式,表明尽管地球化学上相似并且主要由相同的转运蛋白携带,营养金属 (Zn) 与非必需金属 (Cd) 的处理方式不同。高 Zn 和 Cd 下调 PtHMA4(根),上调 PtZIP1(叶),表明它们参与了杨树中两种金属的运输。PtMT2b 在菌根中高度上调,并增强了转化酵母的 Cd 耐受性。R.informalis 减少了 Cd 向杨树枝条的转移,但不影响 Zn 的分配。观察到的基因表达模式提供了对 AMF 共生影响的杨树微量元素吸收/易位动态背后机制的一瞥。PtMT2b 在菌根中高度上调,并增强了转化酵母的 Cd 耐受性。R.informalis 减少了 Cd 向杨树枝条的转移,但不影响 Zn 的分配。观察到的基因表达模式提供了对 AMF 共生影响的杨树微量元素吸收/易位动态背后机制的一瞥。PtMT2b 在菌根中高度上调,并增强了转化酵母的 Cd 耐受性。R.informalis 减少了 Cd 向杨树枝条的转移,但不影响 Zn 的分配。观察到的基因表达模式提供了对 AMF 共生影响的杨树微量元素吸收/易位动态背后机制的一瞥。
更新日期:2020-03-01
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