In this study, a new SYBR Green qPCR (qRT-PCR) and a nested RT-PCR (nRT-PCR) were developed to detect Potato mop-top virus (PMTV) in potato tuber tissues. The SYBR Green qRT-PCR and nRT-PCR assays were approximately 104- and 103- fold more sensitive than the conventional RT-PCR assay. The progeny tubers derived from PMTV-infected potato tubers were tested by conventional RT-PCR, SYBR Green qRT-PCR and nRT-PCR assays. Of the 17 samples, 9 (52.9%) were positive for PMTV by conventional RT-PCR, 11 (64.7%) were positive by nRT-PCR, and 17 (100%) were positive by SYBR Green qRT-PCR. Compared to nRT-PCR, SYBR Green qRT-PCR was showed to be more sensitive. The progeny plants exhibited foliar symptoms including chlorosis and reduction in leaf size when the PMTV-positive tubers were planted in a growth chamber at 20-22 °C. These findings suggest that PMTV has been passed on to the progeny plants and tubers.

中文翻译：

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开发SYBR Green实时PCR和巢式RT-PCR以检测马铃薯拖把病毒（PMTV）并在受PMTV感染的马铃薯块茎衍生的后代块茎中进行病毒调查。

在这项研究中，开发了一种新的SYBR Green qPCR（qRT-PCR）和嵌套RT-PCR（nRT-PCR）来检测马铃薯块茎组织中的马铃薯拖把顶部病毒（PMTV）。SYBR Green qRT-PCR和nRT-PCR分析的灵敏度比常规RT-PCR分析高出约104倍和103倍。通过常规RT-PCR，SYBR Green qRT-PCR和nRT-PCR测定法测试了受PMTV感染的马铃薯块茎的后代块茎。在17个样本中，常规RT-PCR对PMTV呈阳性反应的有9个（52.9％），nRT-PCR对PMTV呈阳性的有11个（64.7％），而SYBR Green qRT-PCR对17个（100％）呈阳性。与nRT-PCR相比，SYBR Green qRT-PCR显示出更高的灵敏度。当将PMTV阳性块茎种植在20-22°C的生长室中时，后代植物表现出叶片症状，包括萎黄和叶尺寸减小。