Anaplasmosis is a tick-borne rickettsial disease caused by Anaplasma marginale, A. centrale, A. phagocytophilum, A. bovis, A. ovis and A. platys. Understanding the phylogenetic relations among these species is fundamental to perform an accurate identification and an informative intra-specific analysis. Heat shock groESL operon is frequently employed in phylogenetic analysis of Anaplasma species and, for the most cases, the use of partial sequences of this operon is randomly done without knowing the most appropriate regions to be used either in species attribution or in intra-specific diversity analysis. In this study, on the basis of all fully and nearly complete groESL sequences available in the GenBank, we firstly selected a minimum partial length sequence which allows species delineation and gives a similar topology to that found by analyzing the complete sequence. By using other in silico analyses, we obtained two minimal partial sequences that are the most interesting to describe intra-specific diversity within A. ovis and A. centrale. Our results raise concern on the use of randomly selected partial sequences of groESL operon employed for the detection and the characterization of Anaplasma species and provide additional background about minimum length groESL operon required for Anaplasma species attribution and strains diversity analysis.

中文翻译：

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Anaplasma物种归因和菌株多样性分析所需的最小长度groESL操纵子的计算选择。

质浆病是由无浆质浆菌，中性曲霉，吞噬嗜性曲霉，牛毛线虫，卵形曲霉和板球菌引起的由tick传播的立克次氏病。了解这些物种之间的系统发育关系对于进行准确的鉴定和提供有益的种内分析至关重要。热休克groESL操纵子经常用于无形体物种的系统发育分析，并且在大多数情况下，该操纵子的部分序列的使用是随机进行的，而不知道用于物种归因或种内多样性的最合适区域。分析。在这项研究中，根据GenBank中所有可用的几乎完整的groESL序列，我们首先选择了一个最小的部分长度序列，该序列可以进行物种描述，并提供与通过分析完整序列而发现的拓扑相似的拓扑。通过使用其他in silico分析，我们获得了两个最小的部分序列，这些最有趣的序列描述了A. ovis和A. centrale内的种内多样性。我们的结果引起了人们对使用groESL操纵子的随机选择部分序列进行检测和鉴定无形体种类的关注，并为无性种属归因和菌株多样性分析所需的最小长度的groESL操纵子提供了额外的背景信息。