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Biofabrication of Gelatin Tissue Scaffolds with Uniform Pore Size via Microbubble Assembly
Macromolecular Materials and Engineering ( IF 4.2 ) Pub Date : 2019-08-21 , DOI: 10.1002/mame.201900394
Cem Bayram 1 , Xinyue Jiang 2 , Merve Gultekinoglu 3 , Sukru Ozturk 3 , Kezban Ulubayram 3 , Mohan Edirisinghe 2
Affiliation  

The control of pore size and uniform porosity remains as an important challenge in gelatin scaffolds. The precise control in building blocks of tissue scaffolds without any additional porogen is possible with costly equipment and techniques, though some pre‐requirements for polymeric material, such as photo‐polymerizability or sintering ability, may be needed prior to construction. Herein, a method for the fabrication of gelatin scaffolds with homogenous porosity using simple T‐junction microfluidics is described. The size of the microbubbles is precisely controlled with 5% deviation from the average. Porous gelatin scaffolds are obtained by building‐up the monodispersed microbubbles in dilute cross‐linker solutions. The effect of cross‐linker density on pore diameter is also investigated. After cross‐linking, pore size of the resultant five scaffold groups are precisely controlled as 135 ± 11, 193 ± 11, 216 ± 9, 231 ± 5, and 250 ± 12 µm. Porosity ratios above 65% are achieved in every sample group. According to the cell culture experiments, structures support high cell adhesion, viability, and migration through the porous network via interconnectivity. This study offers a practical and economical approach for the preparation of porous gelatin scaffolds with homogenous porosity which can be utilized in diverse tissue engineering applications.

中文翻译:

通过微泡组装生物制备具有均匀孔径的明胶组织支架。

在明胶支架中,控制孔径和均匀的孔隙率仍然是重要的挑战。使用昂贵的设备和技术可以在没有任何额外致孔剂的情况下精确控制组织支架的组成部分,尽管在构造之前可能需要对聚合材料进行一些预先要求,例如光聚合性或烧结能力。本文描述了一种使用简单的T型接头微流体制备具有均一孔隙率的明胶支架的方法。精确控制微气泡的大小,与平均值的偏差为5%。明胶多孔支架是通过在稀释的交联剂溶液中建立单分散的微泡而获得的。还研究了交联剂密度对孔径的影响。交联后,精确地将所得的五个支架组的孔径控制为135±11、193±11、216±9、231±5和250±12 µm。在每个样品组中,孔隙率均达到65%以上。根据细胞培养实验,结构支持高细胞粘附性,生存力以及通过互连性通过多孔网络的迁移。这项研究为制备具有均匀孔隙率的多孔明胶支架提供了一种实用且经济的方法,该方法可用于多种组织工程应用中。
更新日期:2019-11-08
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