The uses of probiotics-based food supplements are getting emphasis due to their power to ensure better health conditions. Probiotics have diverse and significant applications in the health sector, so probiotic strains require an understanding of the genome level organizations. Probiotics elucidate various functional parameters that control their metabolic functions. In this review, we have compiled aspects of synthetic biology, which are used for the optimization of metabolic processes in probiotics for their use as a supplement in allopathic medicines. Synthetic biology approaches provide information about diverse biosynthetic pathways and also facilitate the novel metabolic engineering approaches for probiotics strain improvement. We have discussed the synthetic biology approaches for producing engineered probiotics via genetic circuits, expression systems, and genome editing tools like CRISPR-Cas and PEVLAB. This review also enlightens future challenges in the development of engineered probiotics.

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使用合成生物学方法的高效工程益生菌：综述。

基于益生菌的食品补充剂的使用正受到重视，因为它们具有确保更好的健康状况的能力。益生菌在卫生领域具有广泛而重要的应用，因此益生菌菌株需要了解基因组水平的组织。益生菌阐明了控制其代谢功能的各种功能参数。在这篇综述中，我们汇总了合成生物学的各个方面，这些方面可用于优化益生菌的代谢过程，从而将其用作同种疗法药物的补充。合成生物学方法可提供有关多种生物合成途径的信息，也有助于改善益生菌菌株的新型代谢工程方法。我们已经讨论了通过遗传途径生产工程益生菌的合成生物学方法，表达系统以及CRISPR-Cas和PEVLAB等基因组编辑工具。这篇评论也启发了工程益生菌开发中的未来挑战。