Aflatoxin B1 (AFB1 ) removing bacterial strains were isolated from different habitats that were easily contaminated by AFB1 . Furthermore, the composition of the fermentation medium and conditions of fermentation process were optimized, including carbon source, nitrogen source, metal ions, temperature, initial pH value, inoculation volume, and culture broth volume. Using coumarin as the sole carbon and energy source, we primarily screened 31 strains, and 10 strains were found to be capable to remove AFB1 . Among them, the highest removal rate of 71.91% appeared in those isolated from rotten wood (poplar). Strains XY1, XY3, and T6 were carried out to identify, and the results were Klebsiella sp., Klebsiella pneumonia, and Pantoea sp., respectively. Corn cob powder and tryptone can significantly increase the AFB1 removal activity of these strains. The AFB1 removal activity of Klebsiella sp.XY1 and K. pneumonia XY3 can be enhanced by Ca2+ , and those of Pantoea sp. T6 can be enhanced by Cu2+ . Temperature and initial pH were positively correlated with the AFB1 removal activity of these strains in a certain range. This study not only provides reference for the screening and application of AFB1 removing bacteria, but also provides a basis for possible application in the food and feed industry.

中文翻译：

---

黄曲霉毒素B1去除三种细菌菌株和优化发酵工艺参数。

从容易被AFB1污染的不同生境中分离出去除黄曲霉毒素B1（AFB1）的细菌菌株。此外，优化了发酵培养基的组成和发酵过程的条件，包括碳源，氮源，金属离子，温度，初始pH值，接种量和培养液体积。使用香豆素作为唯一的碳和能源，我们主要筛选了31株，发现10株能够去除AFB1。其中，从腐朽木（杨树）中分离出来的那些植物的去除率最高，为71.91％。进行菌株XY1，XY3和T6的鉴定，结果分别为克雷伯菌，肺炎克雷伯菌和泛菌。玉米芯粉和胰蛋白can可以显着提高这些菌株的AFB1去除活性。Ca2 +可以增强Klebsiella sp.XY1和肺炎克雷伯菌XY3的AFB1去除活性，而Pantoea sp。可以提高AFB1去除活性。T6可以通过Cu2 +增强。在一定范围内，温度和初始pH值与这些菌株的AFB1去除活性呈正相关。该研究不仅为AFB1去除菌的筛选和应用提供参考，而且为在食品和饲料工业中的可能应用提供了基础。