Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr.,Biotechnology and Applied Biochemistry

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Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr.
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2019-10-17 , DOI: 10.1002/bab.1839
A R Suprun _{1,

2} , Z V Ogneva ₁ , A S Dubrovina ₁ , K V Kiselev _{1,

2}

Affiliation

Stilbenes are natural compounds protecting plants against microbial pathogens and known to possess valuable biologically active properties. In the present study, we established transgenic grapevine callus cell cultures overexpressing three stilbene synthase (STS ) genes of spruce Picea jezoensis PjSTS1a , PjSTS2 , and PjSTS3 . Transformation of Vitis amurensis calli with the PjSTS1a , PjSTS2 , and PjSTS3 genes significantly increased total content of stilbenes in 3.6–6, 2.5–2.9, and 4.1–16.1 times, respectively, in comparison with the control calli. The most pronounced positive effect on the accumulation of stilbenes was observed for the PjSTS3 ‐overexpressing calli where the total content of stilbenes was increased up to 3.1 mg/g DW, and the stilbene production reached 25.4 mg/L. These values were higher than those achieved for the grapevine callus cell cultures overexpressing three STS genes from V. amurensis . Thus, transformation of grapevine cell cultures with spruce STS genes with a relatively low degree of homology to the endogenous VaSTSs is a more effective strategy for induction of plant secondary metabolite biosynthesis than using the grapevine genes for the overexpression experiments.

中文翻译：

云杉PjSTS1a，PjSTS2或PjSTS3基因过表达对紫葡萄愈伤组织培养物中二苯乙烯生物合成的影响。

Stilbenes是保护植物免受微生物病原体侵害的天然化合物，已知具有宝贵的生物活性。在本研究中，我们建立了过表达云杉云杉云杉云杉PjSTS1a，PjSTS2和PjSTS3的三个二苯乙烯合成酶（STS）基因的转基因葡萄愈伤组织细胞培养物。改造山葡萄愈伤组织与PjSTS1a，PjSTS2和PjSTS3与对照愈伤组织相比，这些基因显着提高了芪的总含量，分别为3.6–6、2.5–2.9和4.1–16.1倍。在过表达PjSTS3的愈伤组织中观察到了对斯蒂尔苯累积的最明显的积极影响，其中斯蒂尔苯的总含量增加到3.1 mg / g DW，而二苯乙烯的产量达到25.4 mg / L。这些值比那些小道消息愈伤组织细胞培养过表达3个实现更高的STS从基因山葡萄。因此，用与内源性VaSTS同源性相对较低的云杉STS基因转化葡萄细胞培养物与使用葡萄基因进行过表达实验相比，它是一种诱导植物次生代谢产物生物合成的更有效策略。

更新日期：2019-10-17

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