According to World Health Organization (WHO) report, Mycobacterium tuberculosis H₃₇Rv (M. tuberculosis ) affects one‐third population of the world. Emergence of effective treatment/research against this disease is need of the hour. Therefore, we present some important aspects of Rv3344c, which is a PE_PGRS protein. Evidence shows that PE_PGRS proteins show fibronectin binding activity. This protein has affinity for calcium and also shows motifs of GTP‐binding protein. It also shows the presence of sites for ribose‐5‐phosphate binding and motifs of aspartate‐beta‐semialdehyde dehydrogenase, both of which are involved in amino acid biosynthesis. Thus, this protein might be targeted to block the amino acid biosynthesis in M. tuberculosis . This article takes into consideration some important aspects of Rv3344c protein as its function is still unknown. This study includes retrieval of protein sequence database, multiple sequence alignment, protein–protein interaction, epitope prediction, localization, function prediction, phosphorylation site prediction, model building and its validation, ligand‐binding prediction along with mutational analysis. Hence, this study might be an important step in the development of new drugs and treatment of tuberculosis.

中文翻译：

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使用生物信息学工具分析结核分枝杆菌H37 Rv中Rv3344c中预测的氨基酸生物合成。

根据世界卫生组织（WHO）的报告，结核分枝杆菌H ₃₇ Rv（结核分枝杆菌）影响世界三分之一的人口。对这种疾病的有效治疗/研究的出现是需要小时的。因此，我们提出了Rv3344c的一些重要方面，Rv3344c是一种PE_PGRS蛋白。有证据表明PE_PGRS蛋白具有纤连蛋白结合活性。该蛋白对钙具有亲和力，并且还显示出GTP结合蛋白的基序。它还显示了核糖5磷酸结合位点和天冬氨酸β半醛脱氢酶的基序，这两个都参与氨基酸的生物合成。因此，该蛋白可能被靶向阻断结核分枝杆菌的氨基酸生物合成。。本文考虑了Rv3344c蛋白的一些重要方面，因为其功能仍然未知。这项研究包括蛋白质序列数据库的检索，多序列比对，蛋白质与蛋白质的相互作用，表位预测，定位，功能预测，磷酸化位点预测，模型建立及其验证，配体结合预测以及突变分析。因此，这项研究可能是开发新药和治疗结核病的重要一步。