Erucic (22:1, cisΔ13) and gondoic acids (20:1, cisΔ11) are building blocks obtained from renewable sources for the oleochemical industry. Different biocatalytic strategies for the enrichment of these compounds with high recovery yields were developed in our group. Geotrichum candidum lipases (GCL) strongly discriminate against fatty acids longer than 18 carbon atoms. Thus, GCL‐I and ‐II were investigated using hydrolysis or ethanolysis reactions with Crambe and Camelina oils. Hydrolysis was also studied using fatty acid ethyl esters (FAEE) derived from the corresponding oil. Both isoforms were highly selective; however, interesting differences were observed. Although it has been reported that GCL‐I displays a higher preference toward 18 cisΔ9, which is present in the studied oils at high levels, GCL‐II showed higher enrichment values during hydrolysis independent of the substrate used. Hence, enrichments of 87% (Crambe oil) and 82% (Crambe FAEE) for erucic acid and 50% (Camelina oil) and 45% (Camelina FAEE) for gondoic acid, with recovery values between 89% and 99%, were achieved. On the contrary, the best enzyme for ethanolysis was GCL‐I (82% and 41% for erucic and gondoic acid, respectively). In this case, although GCL‐II also displayed good enrichment and recovery levels (77% and 28%, respectively), they were lower compared to the former reactions. In both ethanolysis reactions, the FAEE fraction contained between 92% and 97% of 18 unsaturated fatty acids.

中文翻译：

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念珠菌脂肪酶I和II催化Crambe和Camelina油中的芥酸和壬二酸脂肪酸的富集

芥酸（22：1，顺式Δ13）和刚丹酸（20：1，顺式Δ11）是从可再生资源中获得的，用于油脂化工的结构单元。我们小组开发了不同的生物催化策略来富集这些化合物，回收率很高。Geotrichum candidum脂肪酶（GCL）强烈区分长于18个碳原子的脂肪酸。因此，通过与Crambe和Camelina的水解或乙醇解反应研究了GCL-I和-II油。还使用衍生自相应油的脂肪酸乙酯（FAEE）对水解进行了研究。两种同工型都具有高度选择性。但是，观察到有趣的差异。尽管据报道，GCL-I对18顺式Δ9表现出更高的偏爱，后者在研究油中的含量很高，但GCL-II在水解过程中显示出更高的富集值，而与所使用的底物无关。因此，87％（富集海甘蓝油）和82％（海甘蓝FAEE）为芥酸和50％（亚麻荠油）和45％（亚麻荠精氨酸的FAEE）回收率在89％至99％之间。相反，用于乙醇分解的最佳酶是GCL-1（芥酸和贡多酸分别为82％和41％）。在这种情况下，尽管GCL-II也显示出良好的富集和回收率水平（分别为77％和28％），但它们比以前的反应要低。在两个乙醇水解反应中，FAEE馏分均包含92％至97％的18种不饱和脂肪酸。