The ratio of oleic acid to the combined value of linoleic and α‐linolenic acids determines the oxidative stability, and the ratio of linoleic acid to α‐linolenic acid is the key to the nutritional value of soybean oil. The present study was conducted to identify genomic regions associated with oleic, linoleic, and α‐linolenic acids in recombinant inbred lines (RIL), developed from LSb1 × NRC7, across 5 cropping years. These RIL were genotyped using 105 polymorphic SSR markers across soybean genome and analyzed for fatty acid composition. SSR markers, namely, Satt245 (LGp M), Satt556 (LGpB2), Sat_042 (LGp C1), Staga002 (LGp D1b), Satt684 (LGp A1), and AI856415 (LGpD1b) showed significant (P < 0.05) association with oleic acid for all the 5 years, though this association was weak in the years when the growing temperature during active seed formation stage was high. Quantitative trait loci (QTL) linked to Satt684 (LGp A1), Satt556 (LGp B2), Sat_042 (LGp C1), and AI856415 (LGp D1b) showed pleiotropic influence on the levels of unsaturated fatty acids. Complementation of favorable QTL from LSb1 and NRC7 generated 60% oleic acid and less than 4% α‐linolenic acid RIL, stable across 5 cropping years. New SSR markers, namely, Satt245, AI856415, and Staga002 identified to be associated with different unsaturated fatty acids may be useful in improving the efficiency of marker‐assisted breeding for enhancing the monounsaturated to polyunsaturated fatty acids ratio of soybean oil.

中文翻译：

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跨越多年生长的重组重组大豆自交系中不饱和脂肪酸生物合成的基因组区域

油酸与亚油酸和α-亚麻酸的结合值的比例决定了氧化稳定性，而亚油酸与α-亚麻酸的比例是决定豆油营养价值的关键。本研究旨在鉴定从LSb1×NRC7开发的重组自交系（RIL）中油酸，亚油酸和α-亚麻酸的基因组区域，历时5年。使用整个大豆基因组中的105个多态性SSR标记对这些RIL进行基因分型，并分析其脂肪酸组成。SSR标记即Satt245（LGp M），Satt556（LGpB2），Sat_042（LGp C1），Staga002（LGp D1b），Satt684（LGp A1）和AI856415（LGpD1b）显示出显着性（P<0.05）在所有的5年中都与油酸相关，尽管在活跃的种子形成阶段的生长温度高的年份中，这种相关性很弱。与Satt684（LGp A1），Satt556（LGp B2），Sat_042（LGp C1）和AI856415（LGp D1b）相关的定量性状基因座（QTL）对不饱和脂肪酸的含量表现出多效性影响。LSb1和NRC7对有利的QTL的补充产生了60％的油酸和少于4％的α-亚麻酸RIL，在5个种植年中保持稳定。新的SSR标记，即Satt245，AI856415和Staga002被确定与不同的不饱和脂肪酸相关，可能有助于提高标记辅助育种的效率，从而提高大豆油中单不饱和脂肪酸与多不饱和脂肪酸的比例。