This study presented a refining process and reported on fatty acid composition and the physicochemical properties of the oil from black soldier fly larvae (BSFL). Crude larvae oil was purified through four steps consisting of degumming, neutralization, bleaching, and deodorization. Optimum degumming conditions that give the highest phospholipid weight and oil consisted of water concentration of 7% (v/v), followed by addition of H₂SO₄ at a concentration of 0.5% (v/v). Optimum conditions for saponification that maximize saponification value and free fatty acid (FFA) value were 0.4 mg NaOH/100 g oil, 1 hour, and 80 °C of NaOH quantity, reaction time, and temperature, respectively. The oil was then dehydrated using 10 mg Na₂SO₄/g oil. The bleaching process that gives maximum oil yield consisted of activated carbon at concentration of 5% (w/w), followed by centrifugation at a speed of 5000 rpm (radius = 86 mm) for 30 min. The contents of lauric acid, linoleic acid, and linolenic acid in purified oil were 28.8%, 11.1%, and 0.4%, respectively. Physicochemical properties of the refined oil included viscosity of 96 ± 0.14 cP (measured at 20 °C), FFA value of 0.45 ± 0.017%, acid value of 0.9 ± 0.043 mg KOH g⁻¹, saponification value of 215.78 mg KOH g⁻¹, iodine value of 53.7 gI₂/100 g, and peroxide index of 133 mEq kg⁻¹.

中文翻译：

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黑兵蝇幼虫油的纯化工艺，理化性质和脂肪酸组成

这项研究提出了一种精炼工艺，并报道了黑战士蝇幼虫（BSFL）的油中脂肪酸组成和理化性质。粗幼虫油通过脱胶，中和，漂白和除臭四个步骤纯化。使磷脂重量和油含量最高的最佳脱胶条件是水的浓度为7％（v / v），然后以0.5％（v / v）的浓度添加H ₂ SO ₄。使皂化值和游离脂肪酸（FFA）值最大化的最佳皂化条件分别是0.4 mg NaOH / 100 g油，1小时和80°C的NaOH量，反应时间和温度。然后将油用10 mg Na ₂ SO ₄脱水/克油。产生最大油收率的漂白过程由浓度为5％（w / w）的活性炭组成，然后以5000 rpm（半径= 86 mm）的速度离心30分钟。纯化油中月桂酸，亚油酸和亚麻酸的含量分别为28.8％，11.1％和0.4％。精制油的理化性质包括粘度为96±0.14 cP（在20°C下测量），FFA值为0.45±0.017％，酸值为0.9±0.043 mg KOH g ^-1，皂化值为215.78 mg KOH g ^-1，53.7的gI碘值₂ / 100g的，和133毫克当量公斤的过氧化指数^-1。