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In vivo Comprehensive Multiphase NMR
Magnetic Resonance in Chemistry ( IF 1.9 ) Pub Date : 2019-04-02 , DOI: 10.1002/mrc.4832
Yalda Liaghati Mobarhan 1 , Ronald Soong 1 , Daniel Lane 1 , Andre J Simpson 1
Affiliation  

Traditionally, due to different hardware requirements, nuclear magnetic resonance (NMR) has developed as two separate fields: one dealing with solids, and one with solutions. Comprehensive multiphase (CMP) NMR combines all electronics and hardware (magic angle spinning [MAS], gradients, high power Radio Frequency (RF) handling, lock, susceptibility matching) into a universal probe that permits a comprehensive study of all phases (i.e., liquid, gel‐like, semisolid, and solid), in intact samples. When applied in vivo, it provides unique insight into the wide array of bonds in a living system from the most mobile liquids (blood, fluids) through gels (muscle, tissues) to the most rigid (exoskeleton, shell). In this tutorial, the practical aspects of in vivo CMP NMR are discussed including: handling the organisms, rotor preparation, sample spinning, water suppression, editing experiments, and finishes with a brief look at the potential of other heteronuclei (2H, 15N, 19F, 31P) for in vivo research. The tutorial is aimed as a general resource for researchers interested in developing and applying MAS‐based approaches to living organisms. Although the focus here is CMP NMR, many of the approaches can be adapted (or directly applied) using conventional high‐resolution magic angle spinning, and in some cases, even standard solid‐state NMR probes.

中文翻译:

体内综合多相核磁共振

传统上,由于硬件要求不同,核磁共振 (NMR) 已发展为两个独立的领域:一个处理固体,另一个处理溶液。综合多相 (CMP) NMR 将所有电子和硬件(魔角旋转 [MAS]、梯度、高功率射频 (RF) 处理、锁定、磁化率匹配)结合到一个通用探头中,允许对所有相位进行全面研究(即,液体、凝胶状、半固体和固体),在完整样品中。当应用于体内时,它提供了对生命系统中从最易流动的液体(血液、体液)到凝胶(肌肉、组织)到最坚硬(外骨骼、外壳)的各种键的独特见解。在本教程中,讨论了体内 CMP NMR 的实际方面,包括:处理生物体、转子制备、样品旋转、水抑制、编辑实验,并简要介绍了其他异核(2H、15N、19F、31P)在体内研究中的潜力。本教程旨在为有兴趣开发基于 MAS 的方法并将其应用于生物体的研究人员提供一般资源。尽管这里的重点是 CMP NMR,但许多方法都可以使用传统的高分辨率魔角旋转来调整(或直接应用),在某些情况下,甚至可以使用标准的固态 NMR 探针。
更新日期:2019-04-02
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