当前位置：
X-MOL 学术
›
J. Mol. Recognit.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Eriodictyol and naringenin inhibit the formation of AGEs: An in vitro and molecular interaction study.
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2019-10-21 , DOI: 10.1002/jmr.2814 Jianli Liu 1 , Zhijun Yang 1 , Ye Cheng 1 , Qiong Wu 2 , Yin He 1 , Qijiu Li 1 , Xiangyu Cao 1
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2019-10-21 , DOI: 10.1002/jmr.2814 Jianli Liu 1 , Zhijun Yang 1 , Ye Cheng 1 , Qiong Wu 2 , Yin He 1 , Qijiu Li 1 , Xiangyu Cao 1
Affiliation
|
Maillard reaction occurs between the carbonyl group of reducing sugars and the free amino groups of protein, which eventually results in the formation and accumulation of advanced glycation end products (AGEs) irreversibly. Excessive production of AGEs is associated with many diseases, such as Alzheimer disease, neuropathy, retinopathy, and nephropathy. In this study, the effects of eriodictyol and naringenin on the inhibition of AGEs were studied with bovine serum albumin (BSA)-methylglyoxal (MGO) model by spectroscopic techniques and molecular docking methods. The fluorescence spectroscopy results suggested that eriodictyol and naringenin could inhibit the formation of AGEs. Circular dichroism (CD) studies indicated that eriodictyol and naringenin could stabilize the structure of BSA and inhibit the formation of AGEs. The molecular docking results demonstrated that eriodictyol formed two hydrogen bonds with Lys 350 and Leu 480 and the main forces were hydrogen bonding and hydrophobic interactions. However, naringenin interacted with Arg 484 of BSA, and the main force was hydrophobic interaction. It can be concluded that eriodictyol and naringenin can inhibit the formation of AGEs and eriodictyol has stronger inhibitory activity of AGEs than that of naringenin, which is probably due to the additional hydroxyl group in the position C-3' of B ring of eriodictyol.
中文翻译：
Eriodictyol和naringenin抑制AGEs的形成：一项体外和分子相互作用研究。
美拉德反应发生在还原糖的羰基和蛋白质的游离氨基之间，最终导致不可逆地高级糖基化终产物（AGEs）的形成和积累。AGEs的过量产生与许多疾病有关，例如阿尔茨海默氏病，神经病，视网膜病和肾病。在这项研究中，通过光谱技术和分子对接方法，用牛血清白蛋白（BSA）-甲基乙二醛（MGO）模型研究了雌雄醇和柚皮苷对AGEs的抑制作用。荧光光谱结果表明，雌黄和柚皮苷可以抑制AGEs的形成。圆二色性（CD）研究表明，雌黄醇和柚皮苷可以稳定BSA的结构并抑制AGEs的形成。分子对接结果表明，香酚与Lys 350和Leu 480形成两个氢键，主要作用力是氢键和疏水相互作用。然而，柚皮苷与BSA的Arg 484相互作用，其主要作用力是疏水相互作用。可以得出结论：雌雄醇和柚皮苷可以抑制AGEs的形成，而雌雄醇比naringenin具有更强的AGEs抑制活性，这可能是由于雌雄醇B环C-3'位置有额外的羟基。
更新日期：2019-12-13
中文翻译：
Eriodictyol和naringenin抑制AGEs的形成：一项体外和分子相互作用研究。
美拉德反应发生在还原糖的羰基和蛋白质的游离氨基之间，最终导致不可逆地高级糖基化终产物（AGEs）的形成和积累。AGEs的过量产生与许多疾病有关，例如阿尔茨海默氏病，神经病，视网膜病和肾病。在这项研究中，通过光谱技术和分子对接方法，用牛血清白蛋白（BSA）-甲基乙二醛（MGO）模型研究了雌雄醇和柚皮苷对AGEs的抑制作用。荧光光谱结果表明，雌黄和柚皮苷可以抑制AGEs的形成。圆二色性（CD）研究表明，雌黄醇和柚皮苷可以稳定BSA的结构并抑制AGEs的形成。分子对接结果表明，香酚与Lys 350和Leu 480形成两个氢键，主要作用力是氢键和疏水相互作用。然而，柚皮苷与BSA的Arg 484相互作用，其主要作用力是疏水相互作用。可以得出结论：雌雄醇和柚皮苷可以抑制AGEs的形成，而雌雄醇比naringenin具有更强的AGEs抑制活性，这可能是由于雌雄醇B环C-3'位置有额外的羟基。
京公网安备 11010802027423号