Supercritical fluid chromatography (SFC) has experienced a particular revival in recent years thanks to the development of robust and efficient commercial systems. Because of its physico-chemical properties, supercritical carbon dioxide (CO2 ) mixed with cosolvents and additives is particularly suitable for SFC to allow the elution of compounds of different polarities and more particularly complex lipids. Hyphenation with mass spectrometry (MS) is increasingly described in the literature but still requires many further developments in order to be as user-friendly as coupling with liquid chromatography. The basic concepts of SFC and MS hyphenation will be first considered. Then a representative example of method development in lipidomics will be introduced. In conclusion, the challenges and future needs in this field of research will be discussed.

中文翻译：

---

超临界流体色谱与质谱联用，用于脂质组学。

近年来，由于开发了强大而高效的商业系统，超临界流体色谱（SFC）经历了特别的复兴。由于其物理化学特性，与助溶剂和添加剂混合的超临界二氧化碳（CO2）特别适用于SFC，以洗脱不同极性的化合物，尤其是复杂的脂质。质谱联用技术（MS）越来越多地被描述，但是仍然需要许多进一步的发展，以便与液相色谱联用一样易于使用。首先将考虑SFC和MS断字的基本概念。然后，将介绍脂质组学方法开发的代表性示例。总之，将讨论该研究领域中的挑战和未来需求。