当前位置: X-MOL 学术Cytom. Part B Clin. Cytom. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparison of Volumetric and Bead-Based Counting of CD34 Cells by Single-Platform Flow Cytometry.
Cytometry Part B: Clinical Cytometry ( IF 2.3 ) Pub Date : 2019-02-20 , DOI: 10.1002/cyto.b.21773
Luisa Saraiva 1 , Lili Wang 2 , Martin Kammel 3 , Andreas Kummrow 3 , Eleanor Atkinson 4 , Ji Youn Lee 5 , Burhanettin Yalcinkaya 6 , Muslum Akgöz 6 , Jana Höckner 7 , Andreas Ruf 7 , Andrea Engel 8 , Yu-Zhong Zhang 9 , Orla O'Shea 10 , Maria Paola Sassi 11 , Carla Divieto 11 , Tamara Lekishvili 12 , Jonathan Campbell 12 , Yingying Liu 13 , Jing Wang 13 , Richard Stebbings 1 , Adolfas K Gaigalas 2 , Peter Rigsby 4 , Jörg Neukammer 3 , Sandrine Vessillier 1
Affiliation  

BACKGROUND Over 2,000 people a year in the United Kingdom need a bone marrow or blood stem cell transplant. It is important to accurately quantify the hematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system. However, they are present at low frequency, which complicates accurate quantification. The current gold standard method is single-platform flow cytometry using internal reference counting beads to determine the concentration of CD34 cells. However, volumetric flow cytometers have the ability to measure the acquisition volume, which removes the need for reference beads for calculation of cell concentrations. METHOD In this study, we compared both methods for calculating CD34 cell concentrations in volumetric cytometers, using either the volume reading or the number of reference beads for calculation. In addition, the uncertainty of measurement for each method was estimated. RESULTS The results show that both methods have similar uncertainties of measurement. Regression analysis showed low to no statistical difference in CD34 cell concentrations obtained with each method. CONCLUSIONS Overall, this study suggests that the volumetric method is a valid approach but that the adoption of this technology may be hindered without some form of external calibration of volume readings to increase confidence in the measurement. © 2019 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.

中文翻译:

通过单平台流式细胞术比较CD34细胞的体积和基于珠的计数。

背景技术在英国,每年有超过2000人需要骨髓或血液干细胞移植。准确定量造血干细胞以预测移植能否成功补充免疫系统非常重要。但是,它们以低频出现,这使精确定量变得复杂。当前的金标准方法是使用内部参考计数珠确定CD34细胞浓度的单平台流式细胞术。然而,体积流式细胞仪具有测量采集体积的能力,这消除了用于计算细胞浓度的参考珠的需求。方法在这项研究中,我们比较了两种在容积式细胞仪中计算CD34细胞浓度的方法,使用体积读数或参考珠的数量进行计算。另外,估计每种方法的测量不确定度。结果结果表明两种方法的测量不确定度相似。回归分析显示,每种方法获得的CD34细胞浓度低至无统计学差异。结论总体而言,这项研究表明,体积法是一种有效的方法,但是如果不对体积读数进行某种形式的外部校准以增加对测量的信心,则可能会阻碍采用该技术。©2019作者。细胞计数法B部分:Wiley Periodicals,Inc.代表国际临床细胞计数学会出版的临床细胞计数法。结果结果表明两种方法的测量不确定度相似。回归分析显示,每种方法获得的CD34细胞浓度低至无统计学差异。结论总体而言,这项研究表明,体积法是一种有效的方法,但是如果不对体积读数进行某种形式的外部校准以增加对测量的信心,则可能会阻碍采用该技术。©2019作者。细胞计数法B部分:Wiley Periodicals,Inc.代表国际临床细胞计数学会出版的临床细胞计数法。结果结果表明两种方法的测量不确定度相似。回归分析显示,每种方法获得的CD34细胞浓度低至无统计学差异。结论总体而言,这项研究表明,体积法是一种有效的方法,但是如果不对体积读数进行某种形式的外部校准以增加对测量的信心,则可能会阻碍采用该技术。©2019作者。细胞计数法B部分:Wiley Periodicals,Inc.代表国际临床细胞计数学会出版的临床细胞计数法。这项研究表明,体积法是一种有效的方法,但是如果不对体积读数进行某种形式的外部校准以增加对测量的信心,则可能会阻碍采用该技术。©2019作者。细胞计数法B部分:Wiley Periodicals,Inc.代表国际临床细胞计数学会出版的临床细胞计数法。这项研究表明,体积法是一种有效的方法,但是如果不对体积读数进行某种形式的外部校准以增加对测量的信心,则可能会阻碍采用该技术。©2019作者。细胞计数法B部分:Wiley Periodicals,Inc.代表国际临床细胞计数学会出版的临床细胞计数法。
更新日期:2019-02-20
down
wechat
bug