Primary effusion lymphoma (PEL) is a rare B cell lymphoproliferative disorder that arises predominantly in body cavities causing malignant effusions. The incidence of PEL is very low, accounting for approximately 4% of all HIV-associated non-Hodgkin lymphomas. PEL has also been described in elderly patients and after solid-organ transplantation. It is associated in all cases with human herpes virus 8 (HHV8). We describe a case of PEL in a 88-year-old HIV-negative woman who presented with progressive dyspnea and moderate right-sided pleural effusion without significant lymphadenopathies or other effusions. The cytological study of the pleural fluid revealed a dense proliferation of large plasmablastic cells. A six-color multiparametric flow cytometry immunophenotyping study was carried out, and revealed 45% of large in size and high cellular complexity cells positive for CD45 (dim), CD38, CD138, CD30 and HLA-DR; and negative for CD19, CD20, cytoplasmatic CD79a, surface and cytoplasmic light chains Kappa and Lambda, CD3, CD4, CD5, CD7, CD8, CD28, CD56, CD81, and CD117. In situ hybridization for EBV-encoded smalI RNA was negative and immunohistochemistry for Kaposi sarcoma herpesvirus (HHV8) confirmed the diagnosis of PEL. Our results confirm that flow cytometry bring useful data in the diagnosis of large-cell lymphomas involving body cavities. © 2018 International Clinical Cytometry Society.

中文翻译：

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多参数流式细胞术在检测原发渗出性淋巴瘤（PEL）中的实用性。

原发性积液性淋巴瘤（PEL）是一种罕见的B细胞淋巴增生性疾病，主要发生在体腔中，引起恶性积液。PEL的发生率非常低，约占所有与HIV相关的非霍奇金淋巴瘤的4％。在老年患者和实体器官移植后也已描述了PEL。在所有情况下，它都与人类疱疹病毒8（HHV8）相关。我们描述了一个88岁的HIV阴性女性中的PEL病例，该女性表现为进行性呼吸困难和中度右侧胸腔积液，而没有明显的淋巴腺病或其他积液。胸膜液的细胞学研究显示大型成浆细胞密集增殖。进行了六色多参数流式细胞术免疫表型研究，并发现在CD45（dim），CD38，CD138，CD30和HLA-DR阳性的大型和高细胞复杂性细胞中占45％；CD19，CD20，细胞质CD79a，表面和细胞质轻链Kappa和Lambda，CD3，CD4，CD5，CD7，CD8，CD28，CD56，CD81和CD117阴性。EBV编码的smalI RNA的原位杂交阴性，卡波西肉瘤疱疹病毒（HHV8）的免疫组织化学证实了PEL的诊断。我们的结果证实，流式细胞术为涉及体腔的大细胞淋巴瘤的诊断提供了有用的数据。©2018国际临床细胞计量学会。EBV编码的smalI RNA的原位杂交阴性，卡波西肉瘤疱疹病毒（HHV8）的免疫组织化学证实了PEL的诊断。我们的结果证实，流式细胞术为涉及体腔的大细胞淋巴瘤的诊断提供了有用的数据。©2018国际临床细胞计量学会。EBV编码的smalI RNA的原位杂交阴性，卡波西肉瘤疱疹病毒（HHV8）的免疫组织化学证实了PEL的诊断。我们的结果证实，流式细胞术为涉及体腔的大细胞淋巴瘤的诊断提供了有用的数据。©2018国际临床细胞计量学会。