Severe injury and hemorrhagic shock (HS) result in multiple changes to hematopoietic differentiation, which contribute to the development of immunosuppression and multiple organ failure (MOF). Understanding the changes that take place during the acute injury phase may help predict which patients will develop MOF and provide potential targets for therapy. Obtaining bone marrow from humans during the acute injury phase is difficult so published data are largely derived from peripheral blood samples, which infer bone marrow changes that reflect the sustained inflammatory response. This preliminary and opportunistic study investigated leucopoietic changes in rat bone marrow 6 h following traumatic injury and HS. Terminally anesthetized male Porton Wistar rats were allocated randomly to receive a sham operation (cannulation with no injury) or femoral fracture and HS. Bone marrow cells were flushed from rat femurs and immunophenotypically stained with specific antibody panels for lymphoid (CD45R, CD127, CD90, and IgM) or myeloid (CD11b, CD45, and RP-1) lineages. Subsequently, cell populations were fluorescence-activated cell sorted for morphological assessment. Stage-specific cell populations were identified using a limited number of antibodies, and leucopoietic changes were determined 6 h following trauma and HS. Myeloid subpopulations could be identified by varying levels CD11b expression, CD45, and RP-1. Trauma and HS resulted in a significant reduction in total CD11b + myeloid cells including both immature (RP-1(-)) and mature (RP-1+) granulocytes. Multiple B-cell lymphoid subsets were identified. The total percentage of CD90+ subsets remained unchanged following trauma and HS, but there was a reduction in the numbers of maturing CD90(-) cells suggesting movement into the periphery. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

中文翻译：

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大鼠骨髓中造血种群的流式细胞仪分析。创伤和失血性休克的影响。

严重伤害和失血性休克（HS）会导致造血分化的多种变化，从而导致免疫抑制和多器官功能衰竭（MOF）的发展。了解急性损伤阶段发生的变化可能有助于预测哪些患者会发展为MOF并提供潜在的治疗目标。在急性损伤阶段很难从人类获得骨髓，因此已发表的数据主要来自外周血样本，这些样本推断出反映持续炎症反应的骨髓变化。这项初步的机会性研究调查了创伤性损伤和HS后6 h大鼠骨髓的白细胞改变。将最终麻醉的雄性Porton Wistar大鼠随机分配进行假手术（无损伤插管）或股骨骨折和HS。从大鼠股骨冲洗骨髓细胞，并用针对淋巴样（CD45R，CD127，CD90和IgM）或骨髓（CD11b，CD45和RP-1）谱系的特异性抗体板进行免疫表型染色。随后，将细胞群体进行荧光激活的细胞分选以进行形态学评估。使用有限数量的抗体鉴定了阶段特异性细胞群，并在创伤和HS后6小时确定了白细胞变化。骨髓亚群可以通过不同水平的CD11b表达，CD45和RP-1来鉴定。创伤和HS导致CD11b +骨髓细胞总数显着减少，包括未成熟（RP-1（-））和成熟（RP-1 +）粒细胞。确定了多个B细胞淋巴样亚群。创伤和HS后，CD90 +子集的总百分比保持不变，但成熟的CD90（-）细胞数量减少，表明其移动到外周。©2019作者。细胞计数法A部分，由Wiley Periodicals，Inc.代表国际细胞计数发展协会出版。