This 20-color flow cytometry panel was designed to resolve the cellular heterogeneity of antigen-presenting cells and was optimized for lymph node tissue. Reagents were carefully selected and optimized for identification of B cells (B220), neutrophils (Ly6G), monocytes and macrophages (Ly6C, CD169, F4/80), and dendritic cells (XCR1, CD172a, CD11c, I-A/I-E, CD24, CD64, pDCA-1, CD103, CD11b). Inclusion of additional functional markers involved in cell migration (CCR7), co-stimulation (CD80), and adhesion (ICAM-1) enabled further phenotypic characterization. Finally, this panel has been tested and is compatible with fluorescently labeled antigens such as Alexa Fluor 488 (Ax488) for the study of antigen-bearing cells in vivo. Published 2019. This article is a U.S. Government work and is in the public domain in the USA.

中文翻译：

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OMIP-061：20色流式细胞仪面板，用于鼠抗原呈递细胞的高维表征。

该20色流式细胞仪设计用于解决抗原呈递细胞的细胞异质性，并针对淋巴结组织进行了优化。仔细选择和优化试剂以鉴定B细胞（B220），中性粒细胞（Ly6G），单核细胞和巨噬细胞（Ly6C，CD169，F4 / 80）和树突状细胞（XCR1，CD172a，CD11c，IA / IE，CD24，CD64 ，pDCA-1，CD103，CD11b）。包含参与细胞迁移（CCR7），共刺激（CD80）和粘附（ICAM-1）的其他功能性标记可实现进一步的表型鉴定。最后，该板已经过测试，并且与荧光标记的抗原（例如Alexa Fluor 488（Ax488））兼容，可用于体内研究带有抗原的细胞。出版于2019年。本文是美国政府的工作，在美国属于公共领域。