Cancer is a big threat to human life. Asia has about 60% of the global population and accounts for half of global cancer incidence and mortality. Circulating tumor cells (CTCs) have been a good biomarker for cancer diagnosis, staging, and prognosis. Conventional detection methods of CTCs require drawing blood. It may disturb the biological environment and limited real-time monitoring. in vivo flow cytometry (IVFC) is a burgeoning technique that allows noninvasive detection of CTCs in vivo. Here, we review the technical development of IVFC based on various contrast principles, including fluorescence IVFC, photoacoustic IVFC, imaging IVFC, and label-free IVFC. This powerful tool has been applied widely in many areas of cancer-related studies, especially the CTC studies. We review applications of IVFC in preclinical studies on prevalent cancers in Asia, including liver cancer, blood cancer, and so forth. Other cancer-related studies in breast cancer, prostate cancer, cancer-related stem cell research and drug studies are also reviewed. © 2019 International Society for Advancement of Cytometry.

中文翻译：

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体内流式细胞术在癌症研究中的进展。

癌症是对人类生命的重大威胁。亚洲约占全球人口的60％，占全球癌症发病率和死亡率的一半。循环肿瘤细胞（CTC）已成为癌症诊断，分期和预后的良好生物标记。传统的四氯化碳检测方法需要抽血。它可能会干扰生物环境并限制实时监控。体内流式细胞术（IVFC）是一种新兴技术，允许在体内无创检测CTC。在这里，我们回顾基于各种对比原理的IVFC的技术发展，包括荧光IVFC，光声IVFC，成像IVFC和无标签IVFC。这个功能强大的工具已广泛应用于癌症相关研究的许多领域，尤其是CTC研究。我们回顾了IVFC在亚洲流行癌症（包括肝癌，血液癌等）的临床前研究中的应用。还回顾了乳腺癌，前列腺癌，癌症相关干细胞研究和药物研究中的其他与癌症相关的研究。©2019国际细胞计数学会。