CEH-60/PBX regulates vitellogenesis and cuticle permeability through intestinal interaction with UNC-62/MEIS in Caenorhabditis elegans.,PLOS Biology

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CEH-60/PBX regulates vitellogenesis and cuticle permeability through intestinal interaction with UNC-62/MEIS in Caenorhabditis elegans.
PLOS Biology ( IF 7.8 ) Pub Date : 2019-11-01 , DOI: 10.1371/journal.pbio.3000499
Pieter Van de Walle ₁ , Ellen Geens ₁ , Geert Baggerman _{2,

3} , Francisco José Naranjo-Galindo ₁ , Peter Askjaer ₄ , Liliane Schoofs ₁ , Liesbet Temmerman ₁

Affiliation

The onset of sexual maturity involves dramatic changes in physiology and gene expression in many animals. These include abundant yolk protein production in egg-laying species, an energetically costly process under extensive transcriptional control. Here, we used the model organism Caenorhabditis elegans to provide evidence for the spatiotemporally defined interaction of two evolutionarily conserved transcription factors, CEH-60/PBX and UNC-62/MEIS, acting as a gateway to yolk protein production. Via proteomics, bimolecular fluorescence complementation (BiFC), and biochemical and functional readouts, we show that this interaction occurs in the intestine of animals at the onset of sexual maturity and suffices to support the reproductive program. Our electron micrographs and functional assays provide evidence that intestinal PBX/MEIS cooperation drives another process that depends on lipid mobilization: the formation of an impermeable epicuticle. Without this lipid-rich protective layer, mutant animals are hypersensitive to exogenous oxidative stress and are poor partners for mating. Dedicated communication between the hypodermis and intestine in C. elegans likely supports these physiological outcomes, and we propose a fundamental role for the conserved PBX/MEIS interaction in multicellular signaling networks that rely on lipid homeostasis.

中文翻译：

CEH-60 / PBX通过与秀丽隐杆线虫的UNC-62 / MEIS肠道相互作用来调节卵黄发生和表皮通透性。

性成熟的开始涉及许多动物的生理和基因表达的巨大变化。这些措施包括在产卵物种中产生大量卵黄蛋白，这是在广泛的转录控制下耗能巨大的过程。在这里，我们使用模型生物秀丽隐杆线虫（Caenorhabditis elegans）为两个进化保守的转录因子CEH-60 / PBX和UNC-62 / MEIS时空定义的相互作用提供了证据，CEV-60 / PBX和UNC-62 / MEIS是卵黄蛋白生产的门户。通过蛋白质组学，双分子荧光互补（BiFC）以及生化和功能读数，我们表明这种相互作用发生在性成熟期的动物肠道中，足以支持生殖程序。我们的电子显微照片和功能测定法提供了证据，表明肠道PBX / MEIS的合作推动了另一个依赖脂质动员的过程：不可渗透表皮的形成。没有这种富含脂质的保护层，突变动物对外源性氧化应激非常敏感，并且是交配的不良伴侣。秀丽隐杆线虫的皮下组织和肠道之间的专用通讯可能支持这些生理结果，并且我们提出了保守的PBX / MEIS相互作用在依赖脂质稳态的多细胞信号网络中的基本作用。

更新日期：2019-12-03

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