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Altered plant organogenesis under boron deficiency is associated with changes in high-mannose N-glycan profile that also occur in animals
Journal of Plant Physiology ( IF 4.0 ) Pub Date : 2019-12-01 , DOI: 10.1016/j.jplph.2019.153058
María Reguera 1 , Isidro Abreu 1 , Carlos Sentís 1 , Ildefonso Bonilla 1 , Luis Bolaños 1
Affiliation  

Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems. Both organs show an alteration of cell differentiation that result in the development of tumor-like structures. The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG). The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation. Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development. When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells. Additionally, when B was removed at post-hatching, larvae undergo aberrant organogenesis. Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis. Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.

中文翻译:


缺硼下植物器官发生的改变与高甘露糖 N-聚糖谱的变化有关,这种变化也发生在动物身上



硼 (B) 缺乏会影响豌豆根瘤和拟南芥根分生组织的发育。两个器官都显示出细胞分化的改变,导致肿瘤样结构的发展。事实上,植物中的 B 不仅能够与细胞壁的成分相互作用,而且还能够与膜相关的糖复合物相互作用,这促使我们分析高甘露糖型 N-聚糖 (HMNG) 的变化。伴刀豆球蛋白 A 的亲和印迹揭示了 B 剥夺下根瘤和根的早期发育过程中 N-糖基化模式的变化。此外,越来越多的证据表明维生素 B 在动物生理学中的作用,促使我们研究维生素 B 缺乏对斑马鱼发育的影响。当在早期卵裂阶段之前诱导 B 缺乏时,胚胎发育为异常的未分化细胞团。此外,当 B 在孵化后被去除时,幼虫会经历异常的器官发生。与植物中描述的现象类似,B 缺陷斑马鱼幼虫在器官发生之前就发生了 N-糖基化模式的改变。总体而言,这些结果支持 B 在植物和动物中与糖基化相关的常见功能,这可能对发育过程中的细胞信号传导和细胞命运决定很重要。
更新日期:2019-12-01
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