Boron (B) deficiency affects the development of Pisum sativum nodules and Arabidopsis thaliana root meristems. Both organs show an alteration of cell differentiation that result in the development of tumor-like structures. The fact that B in plants is not only able to interact with components of the cell wall but also with membrane-associated glycoconjugates, led us to analyze changes in high mannose type N-glycans (HMNG). The affinoblots with concanavalin A revealed alterations in the N-glycosylation pattern during early development of nodules and roots under B deprivation. Besides, there is increasing evidence of a B role in animal physiology that brought us to investigate the impact of B deficiency on Danio rerio (zebrafish) development. When B deficiency was induced prior to early cleavage stages, embryos developed as an abnormal undifferentiated mass of cells. Additionally, when B was removed at post-hatching, larvae undergo aberrant organogenesis. Resembling the phenomenon described in plants, alteration of the N-glycosylation pattern occurred in B-deficient zebrafish larvae prior to organogenesis. Overall, these results support a common function of B in plants and animals associated with glycosylation that might be important for cell signaling and cell fate determination during development.

中文翻译：

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缺硼下植物器官发生的改变与高甘露糖 N-聚糖谱的变化有关，这种变化也发生在动物身上


硼 (B) 缺乏会影响豌豆根瘤和拟南芥根分生组织的发育。两个器官都显示出细胞分化的改变，导致肿瘤样结构的发展。事实上，植物中的 B 不仅能够与细胞壁的成分相互作用，而且还能够与膜相关的糖复合物相互作用，这促使我们分析高甘露糖型 N-聚糖 (HMNG) 的变化。伴刀豆球蛋白 A 的亲和印迹揭示了 B 剥夺下根瘤和根的早期发育过程中 N-糖基化模式的变化。此外，越来越多的证据表明维生素 B 在动物生理学中的作用，促使我们研究维生素 B 缺乏对斑马鱼发育的影响。当在早期卵裂阶段之前诱导 B 缺乏时，胚胎发育为异常的未分化细胞团。此外，当 B 在孵化后被去除时，幼虫会经历异常的器官发生。与植物中描述的现象类似，B 缺陷斑马鱼幼虫在器官发生之前就发生了 N-糖基化模式的改变。总体而言，这些结果支持 B 在植物和动物中与糖基化相关的常见功能，这可能对发育过程中的细胞信号传导和细胞命运决定很重要。