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Toxicity of the aquatic herbicide, reward®, on the fathead minnow with pulsed-exposure proteomic profile.
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2019-11-01 , DOI: 10.1016/j.cbd.2019.100635 Michael L Moreton 1 , Bonnie P Lo 2 , Denina B D Simmons 3 , Vicki L Marlatt 1
Comparative Biochemistry and Physiology D: Genomics & Proteomics ( IF 2.2 ) Pub Date : 2019-11-01 , DOI: 10.1016/j.cbd.2019.100635 Michael L Moreton 1 , Bonnie P Lo 2 , Denina B D Simmons 3 , Vicki L Marlatt 1
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The objectives of this study were to assess the lethal and sub-lethal effects of the aquatic herbicide commercial formulation, Reward® (373 g/L DB), using application scenarios prescribed by the manufacturer. Specifically, a 14 d period between applications of Reward® in a water body undergoing treatment is required, yet the effects of these 'pulse' exposure scenarios on aquatic wildlife such as fish are unknown. In the first experiment early life stage FHM were exposed to a continuous DB concentrations from 0.105-12.6 mg/L which yielded a larval 7 d LC50 of 2.04 mg/L as well as a significant decrease in body mass (25.0 ± 11.6%) at the 1.18 mg/L Reward® concentration. In a second experiment, FHM larvae were exposed for 24 h and then reared in clean water for 14 d followed by a second 24 h exposure to Reward®. The 16 d LC50 value was 4.19 mg/L. In a third experiment, adult FHM were exposed in a pulse/discontinuous manner to Reward® with a calculated 21 d LC50 value of 6.71 mg/L. No significant changes in gonadosomatic index or fecundity of the F1 generation's hatch success were found when eggs from exposed adults were then reared in clean water. Proteome analyses of whole FHM larvae from the discontinuous/pulse exposure showed the primary gene ontology molecular functions of the proteins in fish exposed to 3.78 mg/L DB that resulted in ~30% mortality with positive or negative differential abundance (p-value < .2) were: structural molecule activity; identical protein binding; structural constituent of cytoskeleton; ion binding; calcium ion binding; cytoskeletal protein binding; actin binding; and, ATP binding. These findings suggest that concentrations causing adverse effects occur above the maximum concentration predicted by the manufacturer when applied according to the label (i.e. >0.37 mg/L).
中文翻译：
水生除草剂奖励在the头min鱼上的毒性，具有脉冲暴露的蛋白质组学特征。
这项研究的目的是使用制造商规定的应用场景，评估水生除草剂商业配方Reward®（373 g / L DB）的致死和亚致死作用。具体来说，在经过处理的水域中，需要在Reward®施用之间间隔14 d，但是尚不清楚这些“脉冲”暴露场景对水生野生生物（例如鱼类）的影响。在第一个实验中，生命早期阶段的FHM暴露于0.105-12.6 mg / L的连续DB浓度下，其幼体7 d LC50为2.04 mg / L，并且体重显着降低（25.0±11.6％）。 Reward®浓度为1.18 mg / L。在第二个实验中，将FHM幼虫暴露24小时，然后在纯净水中饲养14天，然后再暴露24小时。16 d LC50值为4。19毫克/升。在第三个实验中，将成年的FHM以脉冲/不连续方式暴露于Reward®，计算出的21 d LC50值为6.71 mg / L。当将成年受感染的成虫的卵放在干净的水中饲养时，未发现F1代孵化成功的性腺机体指数或繁殖力有显着变化。对不连续/脉冲暴露的整个FHM幼虫的蛋白质组学分析表明，暴露于3.78 mg / L DB的鱼中蛋白质的主要基因本体分子功能导致〜30％的死亡率，正或负差异丰度（p值<。 2）分别是：结构分子的活性；相同的蛋白质结合；细胞骨架的结构成分；离子结合 钙离子结合；细胞骨架蛋白结合；肌动蛋白结合 并且，ATP结合。
更新日期：2019-11-01
中文翻译：
水生除草剂奖励在the头min鱼上的毒性，具有脉冲暴露的蛋白质组学特征。
这项研究的目的是使用制造商规定的应用场景，评估水生除草剂商业配方Reward®（373 g / L DB）的致死和亚致死作用。具体来说，在经过处理的水域中，需要在Reward®施用之间间隔14 d，但是尚不清楚这些“脉冲”暴露场景对水生野生生物（例如鱼类）的影响。在第一个实验中，生命早期阶段的FHM暴露于0.105-12.6 mg / L的连续DB浓度下，其幼体7 d LC50为2.04 mg / L，并且体重显着降低（25.0±11.6％）。 Reward®浓度为1.18 mg / L。在第二个实验中，将FHM幼虫暴露24小时，然后在纯净水中饲养14天，然后再暴露24小时。16 d LC50值为4。19毫克/升。在第三个实验中，将成年的FHM以脉冲/不连续方式暴露于Reward®，计算出的21 d LC50值为6.71 mg / L。当将成年受感染的成虫的卵放在干净的水中饲养时，未发现F1代孵化成功的性腺机体指数或繁殖力有显着变化。对不连续/脉冲暴露的整个FHM幼虫的蛋白质组学分析表明，暴露于3.78 mg / L DB的鱼中蛋白质的主要基因本体分子功能导致〜30％的死亡率，正或负差异丰度（p值<。 2）分别是：结构分子的活性；相同的蛋白质结合；细胞骨架的结构成分；离子结合 钙离子结合；细胞骨架蛋白结合；肌动蛋白结合 并且，ATP结合。
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