Type 2 diabetes (T2D) is the most common endocrine and metabolic disorder, leading to reproductive impairments and infertility in male. Our recent study showed crucial role of adiponectin in the regulation of testicular functions, and the circulating level of adiponectin declines in diabetes. The current study thus aimed to examine the efficacy of adiponectin in improving testicular dysfunction in high-fat diet/streptozotocin-induced T2D mice. T2D was induced in pre-pubertal mice fed with high-fat diet for ∼10 weeks followed by a single dose of streptozotocin. T2D mice showed presence of increased body mass, hyperglycemia, hyperinsulinemia, insulin resistance, increased oxidative stress, and declined serum testosterone compared to vehicle-treated control mice. The spermatogenic, steroidogenic, metabolic, and antioxidative parameters were evaluated in T2D mice treated with adiponectin for both two and four weeks. The exogenous administration of adiponectin to T2D mice showed enhanced serum testosterone and expression of testicular steroidogenic markers proteins, insulin receptor and GLUT8 proteins, increase in intra-testicular concentrations of glucose and lactate and activity of LDH and antioxidant enzymes compared to the levels in untreated T2D mice. This suggests that treatment of adiponectin effectively improves testicular functions by increasing expression of insulin receptor-mediated increased transport of energy substrate (glucose and lactate) and a marked reduction in oxidative stress are the possible mechanism by which adiponectin effectively improves testicular function in T2D mice.

中文翻译：

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脂联素对高脂饮食/链脲佐菌素诱导的2型糖尿病小鼠睾丸损伤的保护作用。

2型糖尿病（T2D）是最常见的内分泌和代谢疾病，导致男性生殖功能障碍和不育。我们最近的研究显示脂联素在调节睾丸功能中的关键作用，以及糖尿病中脂联素的循环水平下降。因此，当前的研究旨在检验脂联素在改善高脂饮食/链脲佐菌素诱导的T2D小鼠睾丸功能障碍中的功效。在高脂饮食喂养约10周的青春期前小鼠中诱导T2D，然后再注射单剂量的链脲佐菌素。与媒介物治疗的对照小鼠相比，T2D小鼠表现出体重增加，高血糖，高胰岛素血症，胰岛素抵抗，氧化应激增加和血清睾丸激素降低的存在。生精，类固醇，代谢，在用脂联素处理2周和4周的T2D小鼠中评估其抗氧化参数。与未处理的T2D相比，脂联素对T2D小鼠的外源给药显示出血清睾丸激素水平提高和睾丸类固醇生成标记蛋白，胰岛素受体和GLUT8蛋白的表达，睾丸内葡萄糖和乳酸浓度以及LDH和抗氧化酶活性的增加。老鼠。这表明脂联素的治疗可通过增加胰岛素受体介导的能量底物（葡萄糖和乳酸）运输的增加而有效地改善睾丸功能，而氧化应激的显着降低是脂联素有效改善T2D小鼠睾丸功能的可能机制。