Heterochiral DNA Strand-Displacement Based on Chimeric d/l-Oligonucleotides.,ACS Synthetic Biology

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Heterochiral DNA Strand-Displacement Based on Chimeric d/l-Oligonucleotides.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2019-11-11 , DOI: 10.1021/acssynbio.9b00335
Brian E Young ₁ , Jonathan T Sczepanski ₁

Affiliation

Heterochiral DNA strand-displacement reactions enable sequence-specific interfacing of oligonucleotide enantiomers, making it possible to interface native d-nucleic acids with molecular circuits built using nuclease-resistant l-DNA. To date, all heterochiral reactions have relied on peptide nucleic acid (PNA), which places potential limits on the scope and utility of this approach. Herein, we now report heterochiral strand-displacement in the absence of PNA, instead utilizing chimeric d/l-DNA complexes to interface oligonucleotides of the opposite chirality. We show that these strand-displacement reactions can be easily integrated into multicomponent heterochiral circuits, are compatible with both DNA and RNA inputs, and can be engineered to function in serum-supplemented medium. We anticipate that these new reactions will lead to a wider application of heterochiral strand-displacement, especially in the design of biocompatible nucleic acid circuits that can reliably operate within living systems.

中文翻译：

基于嵌合d / l-寡核苷酸的异源DNA链置换。

异源DNA链置换反应可实现寡核苷酸对映异构体的序列特异性接口，从而使天然d-核酸与使用耐核酸酶的l-DNA构建的分子回路相连接成为可能。迄今为止，所有的杂手性反应都依赖于肽核酸（PNA），这对这种方法的范围和实用性构成了潜在的限制。在此，我们现在报道在不存在PNA的情况下进行异手性链置换，而不是利用嵌合d / l-DNA复合物来连接具有相反手性的寡核苷酸。我们表明，这些链置换反应可以很容易地集成到多组分的异手性电路中，与DNA和RNA的输入都兼容，并且可以被工程化以在补充血清的培养基中起作用。

更新日期：2019-11-11

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