Direct, NAD(P)H-independent regeneration of Old Yellow Enzymes represents an interesting approach for simplified reaction schemes for the stereoselective reduction of conjugated C=C-double bonds. Simply by illuminating the reaction mixtures with blue light in the presence of sacrificial electron donors enables to circumvent the costly and unstable nicotinamide cofactors and a corresponding regeneration system. In the present study, we characterise the parameters determining the efficiency of this approach and outline the current limitations. Particularly, the photolability of the flavin photocatalyst and the (flavin-containing) biocatalyst represent the major limitation en route to preparative application.

中文翻译：

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黄素酶的光化学再生 - 一个古老的黄色酶案例研究。

旧黄色酶的直接、NAD(P)H 独立再生代表了一种有趣的方法，用于简化反应方案，用于立体选择性还原共轭 C=C-双键。简单地通过在牺牲电子供体存在的情况下用蓝光照射反应混合物能够绕过昂贵且不稳定的烟酰胺辅助因子和相应的再生系统。在本研究中，我们描述了决定这种方法效率的参数并概述了当前的局限性。特别是，黄素光催化剂和（含黄素）生物催化剂的光稳定性代表了制备应用过程中的主要限制。